Influence of Aae Autotransporter Protein on Adhesion and Biofilm Formation by Aggregatibacter actinomycetemcomitans

Autor(es)

Ana Carla Robatto Nunes ; Priscila Larcher Longo ; Marcia Pinto Alves Mayer ^[3]

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 27, n. 3, p. 255-260, 2016-06-00.

Resumo

Abstract The periodontopathogen Aggregatibacter actinomycetemcomitans colonizes oral cavity by binding to and invading epithelial cells as well as by participating in biofilms formed on hard surfaces. Aae, an autotransporter protein, is implicated in bacterial adhesion to epithelial cells. Due to the multiple functions of bacterial autotransporter proteins, this study aimed to evaluate the role of aae in A. actinomycetemcomitans ability to adhere to both saliva-coated hydroxyapatite (SHA) and biofilm. An aae null mutant was constructed. Its hydrophobic properties as well as its ability to adhere to epithelial cells, SHA and to form biofilm were evaluated and compared with the parental strain, A. actinomycetemcomitans VT1169. The aae null mutant showed reduced hydrophobicity, as well as decreased binding to SHA and biofilm formation compared to the parental strain. These data suggest that aae mediates A. actinomycetemcomitans adhesion to epithelial cells and may be involved in biofilm formation and interaction with adsorbed salivary proteins. (AU)

• Processo FAPESP: 03/11172-5 - Avaliacao do polimorfismo do gene aae de actinobacillus actinomycetemcomitans e do papel desempenhado na aderencia bacteriana as celulas epiteliais, na presenca e ausencia da lactoferrina humana..
• Beneficiário: Ana Carla Robatto Nunes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 03/08598-0 - Fatores de virulência de A. actinomycetemcomitans: participação na doença, regulação da expressão, diversidade e resposta imune
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Temático