Genetic diversity of Parides ascanius (Lepidoptera: Papilionidae: Troidini): implications for the conservation of Brazil's most iconic endangered invertebrate species

Autor(es)

Seraphim, N. ^{[1, 2]} ; Barreto, M. A. ^[3] ; Almeida, G. S. S. ^[4] ; Esperanco, A. P. ^[5] ; Monteiro, R. F. ^[5] ; Souza, A. P. ^{[3, 6]} ; Freitas, A. V. L. ^[1] ; Silva-Brandao, K. L. ^[7]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Anim, Cidade Univ Zeferino Vaz, Caixa Postal 6109, BR-13083970 Campinas, SP - Brazil

^[2] Inst Fed Educ Ciencia & Tecnol Sao Paulo, Campus Capivari, Ave Dr Enio Pires de Camargo 2971, BR-13360000 Capivari, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Ctr Biol Mol & Engn Genet, Cidade Univ Zeferino Vaz, BR-13083970 Campinas, SP - Brazil

^[4] Univ Fed Fluminense, Inst Biol, Dept Biol Geral, Caixa Postal 100-436, BR-24020971 Niteroi, RJ - Brazil

^[5] Univ Fed Rio de Janeiro, Inst Biol, Dept Ecol, Lab Ecol Insetos, CP68020, BR-21941590 Rio De Janeiro, RJ - Brazil

^[6] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Vegetal, Cidade Univ Zeferino Vaz, Caixa Postal 6109, BR-13083970 Campinas, SP - Brazil

^[7] Univ Sao Paulo, Lab Melhoramento Plantas, Ctr Energia Nucl Agr, Campus Luiz de Queiroz, Av Centenario 303, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS; v. 17, n. 3, p. 533-546, JUN 2016.
• Citações Web of Science: 3

Resumo

Parides ascanius (Lepidoptera: Papilionidae) is a butterfly endemic to the sand forests ({''}restingas{''}) of one of the most populated areas of Brazil (from Rio de Janeiro state to South Espirito Santo state), and was the first invertebrate officially recognized as being threatened in Brazil. Here we present a panel of eight polymorphic microsatellite loci and partial sequences of mitochondrial gene COI aiming to characterize this butterfly's genetic diversity and understand its distribution among the extant populations. We estimate F-ST metrics, migration rates, cluster assignment, and spatial structure of genetic diversity. F-ST and statistics indicate low genetic structure and no evidence for endogamy, with all populations connected by high migration rates. Seven populations have low permanence rates (68-75 %) with increased migration probabilities for all populations. One population displays higher permanence rate (87.7 %), as the metropolitan matrix isolates it. Spatial analysis shows a global structure around the city of Rio de Janeiro and the Guanabara Bay; assignment analysis recovers six clusters evenly spread among sampled populations. These findings are consistent with a natural scenario of metapopulation dynamics for P. ascanius, with low genetic diversity and no endogamy, but progressively isolated by the metropolitan matrix. Conservation efforts should focus in connecting the isolated population, broaden the searches for new populations, and preserve all extant habitat patches where P. ascanius still occurs. (AU)

• Processo FAPESP: 08/52197-4 - Genomic-assisted breeding of sugarcane: using molecular markers for understanding the genetic architecture of quantitative traits and to implement marker assisted selection
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/50225-3 - História natural, filogenia e conservação de lepidópteros neotropicais
• Beneficiário: André Victor Lucci Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular