Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products

Autor(es)

Guimaraes-Cestaro, Lubiane ^[1] ; Serrao, Jose Eduardo ^[2] ; Message, Dejair ^[3] ; Martins, Marta Fonseca ^[4] ; Teixeira, Erica Weinstein ^[5]

Afiliação do(s) autor(es)

^[1] Univ Fed Vicosa, Entomol, Av Peter Henry Rolfs S-N, BR-36571000 Vicosa, MG - Brazil

^[2] Univ Fed Vicosa, Dept Gen Biol DBG, Av Peter Henry Rolfs S-N, BR-36571000 Vicosa, MG - Brazil

^[3] Fed Rural Univ Semiarid UFERSA, Dept Anim Sci DCAn, Km 47, BR-110 Mossoro, RN - Brazil

^[4] Embrapa Dairy Cattle, Mol Genet Lab, Rua Eugenio do Nascimento 610, BR-36038330 Juiz De Fora, MG - Brazil

^[5] Sao Paulo State Agribusiness Technol Agcy APTA SA, Honey Bee Hlth Lab LASA, Caixa Postal 07, BR-12400970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF HYMENOPTERA RESEARCH; v. 49, p. 43-50, 2016.
• Citações Web of Science: 4

Resumo

Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions. (AU)

• Processo FAPESP: 12/18802-3 - Identificação de patógenos de abelhas em produtos apícolas comercializados no Estado de São Paulo e perfil da sanidade apícola no Vale do Ribeira, SP
• Beneficiário: Érica Weinstein Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular