In vitro maturation alters gene expression in bovine oocytes

Autor(es)

Adona, Paulo R. ^{[1, 2, 3]} ; Leal, Claudia L. V. ^[1] ; Biase, Fernando H. ^[4] ; De Bem, Tiago H. ^[1] ; Mesquita, Ligia G. ^[1] ; Meirelles, Flavio V. ^[1] ; Ferraz, Andre L. ^[5] ; Furlan, Luiz R. ^[6] ; Monzani, Paulo S. ^{[3, 7]} ; Guemra, Samuel ^{[3, 7]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Basicas, Pirassununga, SP - Brazil

^[2] Agr Laffranchi, POB 45, BR-86125000 Tamarana, Parana - Brazil

^[3] Univ Norte Parana, Escola Med Vet, Arapongas, PR - Brazil

^[4] Auburn Univ, Dept Anim Sci, Auburn, AL - USA

^[5] Univ Estadual Mato Grosso Sul, Escola Zootecnia, Aquidauana, MS - Brazil

^[6] Univ Estadual Paulista, Dept Tecnol, Botucatu, SP - Brazil

^[7] Agr Laffranchi, Lab Reprod Anim, Tamarana, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZYGOTE; v. 24, n. 4, p. 624-633, AUG 2016.
• Citações Web of Science: 8

Resumo

Gene expression profiling of in vivo- and in vitro-matured bovine oocytes can identify transcripts related to the developmental potential of oocytes. Nonetheless, the effects of in vitro culturing oocytes are yet to be fully understood. We tested the effects of in vitro maturation on the transcript profile of oocytes collected from Bos taurus indicus cows. We quantified the expression of 1488 genes in in vivo-and in vitro-matured oocytes. Of these, 51 genes were up-regulated, whereas 56 were down-regulated (>= 2-fold) in in vivo-matured oocytes in comparison with in vitro-matured oocytes. Quantitative real-time polymerase chain reaction (PCR) of nine genes confirmed the microarray results of differential expression between in vivo- and in vitro-matured oocytes (EZR, EPN1, PSEN2, FST, IGFBP3, RBBP4, STAT3, FDPS and IRS1). We interrogated the results for enrichment of Gene Ontology categories and overlap with protein-protein interactions. The results revealed that the genes altered by in vitro maturation are mostly related to the regulation of oocyte metabolism. Additionally, analysis of protein-protein interactions uncovered two regulatory networks affected by the in vitro culture system. We propose that the differentially expressed genes are candidates for biomarkers of oocyte competence. In vitro oocyte maturation can affect the abundance of specific transcripts and are likely to deplete the developmental competence. (AU)

• Processo FAPESP: 05/58702-4 - Avaliacao de rnam diferencialmente transcritos em ovocitos maturados in vivo e in vitro para identificacao de possiveis marcadores de competencia ovocitaria.
• Beneficiário: Paulo Roberto Adona
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/59694-5 - Avaliacao de rnam diferencialmente transcritos em ovocitos bovinos maturados in vivo e in vitro para identificacao de possiveis marcadores de competencia ovocitaria.
• Beneficiário: Cláudia Lima Verde Leal
• Modalidade de apoio: Auxílio à Pesquisa - Regular