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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The optical properties of mouse skin in the visible and near infrared spectral regions

Texto completo
Sabino, Caetano P. [1, 2, 3] ; Deana, Alessandro M. [4] ; Yoshimura, Tania M. [3] ; da Silva, Daniela F. T. [4] ; Franca, Cristiane M. [4] ; Hamblin, Michael R. [5, 6, 7] ; Ribeiro, Martha S. [3]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo, SP - Brazil
[3] IPEN CNEN SP, Nucl & Energy Res Inst, Ctr Lasers & Applicat, Sao Paulo, SP - Brazil
[4] Univ Nove de Julho, Biophoton Postgrad Program, Sao Paulo, SP - Brazil
[5] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 - USA
[6] Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 - USA
[7] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Citações Web of Science: 32

Visible and near-infrared radiation is now widely employed in health science and technology. Pre-clinical trials are still essential to allow appropriate translation of optical methods into clinical practice. Our results stress the importance of considering the mouse strain and gender when planning pre-clinical experiments that depend on light-skin interactions. Here, we evaluated the optical properties of depilated albino and pigmented mouse skin using reproducible methods to determine parameters that have wide applicability in biomedical optics. Light penetration depth (delta), absorption (mu(a)), reduced scattering (mu'(s)) and reduced attenuation (mu'(t)) coefficients were calculated using the Kubellca-Munk model of photon transport and spectrophotometric measurements. Within a broad wavelength coverage (400-1400 nm), the main optical tissue interactions of visible and near infrared radiation could be inferred. Histological analysis was performed to correlate the findings with tissue composition and structure. Disperse melanin granules present in depilated pigmented mouse skin were shown to be irrelevant for light absorption. Gender mostly affected optical properties in the visible range due to variations in blood and abundance of dense connective tissue. On the other hand, mouse strains could produce more variations in the hydration level of skin, leading to changes in absorption in the infrared spectral region. A spectral region of minimal light attenuation, commonly referred as the ``optical window{''}, was observed between 600 and 1350 nm. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 14/02564-1 - Tratando a meta-inflamação da síndrome metabólica usando terapia com luz de baixa potência
Beneficiário:Martha Simões Ribeiro
Linha de fomento: Auxílio à Pesquisa - Regular