Noninvasive characterization of metabolites secreted in culture media by bovine embryos during in vitro production

Autor(es)

dos Santos, Erika Cristina ^[1] ; de Lima, Camila Bruna ^[2] ; Annes, Kelly ^[1] ; Milazzotto, Marcella Pecora ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Nat & Human Sci, Rua Santa Adelia 166, Bloco A, Torre 3, S642, Santo Andre, SP - Brazil

^[2] Univ Sao Paulo, Dept Biomed Sci, Ave Prof Lineu Prestes 2415, Edificio ICB 3, Cidade Univ, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METABOLOMICS; v. 12, n. 5 MAY 2016.
• Citações Web of Science: 1

Resumo

Introduction Secreted molecules could be correlated with the potential of embryonic development. The development of new technologies, such as mass spectrometry (MS), has enabled analyzes in culture medium to favor the determination of embryos viability in order to improve embryo selection. Objectives To perform a non-invasive characterization of the secretome of in vitro produced embryos with different kinetics of cleavage and in different stages of development to obtain specific patterns based on embryonic phenotype through MALDI-TOF-MS. Methods Bovine embryos were produced in vitro by standard protocols. The zygotes were transferred to individual culture medium and divided into two groups: Fast {[}4 cells-22 hours past the beginning of culture (hpc)] and Slow (2 cells-22 hpc). Culture media drops were collected at 22, 96 and 168 hpc. Analysis of embryonic secretome was made by MALDI-TOF-MS after extractions of the metabolites. Spectra were acquired in positive ionization mode. Univariate (Fold-change) and multivariate (Partial Least Squares Discriminants Analysis) analyses were performed by the online software Metaboanalyst. Results It was demonstrated that embryos with different kinetics have different spectrometric profiles during embryonic development. Moreover, secreted molecules in each developmental stage are differentially represented in embryos with different kinetics, and are related to specific pathways such as lipid and amino acids metabolism and cell proliferation. Conclusion We propose that the analysis of culture media by MALDI-TOF-MS can be used for qualitative characterization of bovine embryos, allowing the identification of key molecules during in vitro culture. (AU)

• Processo FAPESP: 12/10351-2 - Metabolômica para avaliação não invasiva de embriões bovinos produzidos in vitro
• Beneficiário: Marcella Pecora Milazzotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular