Recovery of normal testicular temperature after scrotal heat stress in rams assessed by infrared thermography and its effects on seminal characteristics and testosterone blood serum concentration

Autor(es):Mostrar menos -

Rodrigues Alves, Maira Bianchi ^[1] ; Cesar de Andrade, Andre Furugen ^[2] ; de Arruda, Rubens Paes ^[3] ; Batissaco, Leonardo ^[1] ; Florez-Rodriguez, Shirley Andrea ^[1] ; Martins de Oliveira, Bruna Marcele ^[1] ; Torres, Mariana Andrade ^[2] ; Lanconi, Renata ^[3] ; Ravagnani, Gisele Mouro ^[2] ; do Prado Filho, Roberto Romano ^[1] ; Vellone, Vinicius Silva ^[1] ; de Agostini Losano, Joao Diego ^[4] ; Franci, Celso Rodrigues ^[5] ; Nichi, Marcilio ^[4] ; Carvalho Celeghini, Eneiva Carla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Ctr Biotechnol Anim Reprod, Lab Teaching & Res Pathol Reprod, Dept Anim Reprod, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl & Embryo Technol, Pirassununga, SP - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Ctr Biotechnol Anim Reprod, Lab Semen Biotechnol & Androl, Dept Anim Reprod, Pirassununga, SP - Brazil

^[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Lab Androl, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Fac Med, Dept Physiol, Lab Neuroendocrinol & Reprod, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 86, n. 3, p. 795-805, AUG 2016.
• Citações Web of Science: 8

Resumo

Reestablishment of testicular normal temperature after testicular heat stress is unknown and its effect varies widely. The aim of this study was to investigate the impact of scrotal insulation (IN) on testicular temperature and its relation to semen quality and testosterone blood serum concentration. For this, 33 rams were used; 17 submitted to IN for 72 hours (using bags involving the testes) and 16 not submitted to IN (control group). The experiment was performed between August and December 2013 in Pirassununga, Brazil (21 degrees 56{''}13{''} South/47 degrees 28'24{''} West). Seminal characteristics, testosterone blood serum concentration, rectal temperature (RT), respiratory frequency, scrotal superficies mean temperature (SSMT), and eye area mean temperature (EAMT) were analyzed 7 days before IN and 21, 35, 49, 63, and 90 days afterward. Scrotal superficies mean temperature and EAMT were measured by thermography camera FUR T620. Testosterone was evaluated by, radioimmunoassay. Analysis of variance was used to determine the main effects of treatment, time, and treatment-by-time interaction using PROC MIXED of SAS software adding command REPEAT. Pearson correlation test was used to verify correlation between SSMT, EAMT, RT, and respiratory frequency. Significant difference was considered when P < 0.05. At the end of IN, SSMT was higher (P < 0.05) in insulated group (32.26 +/- 0.19 degrees C) than in control group (30.58 +/- 0.18 degrees C), and the difference between rectal and testicular (deduced from SSMT) temperatures was 1.12 degrees C; in the other times of the evaluation this difference was between 2.91 and 4.25 degrees C in IN group. Scrotal superficies mean temperature was reestablished 24 hours after IN. Rectal temperature and EAMT presented correlation (r = 0:59; P < 0.0001). There was time -by -treatment interaction for total sperm (P = 0.0038) and progressive motility (P = 0.01), abnormal spermatozoa (P < 0.0001), membranes integrity (P < 0.0001), induced thiobarbituric acid reactive substances (TBARSs; P = 0.05), and DNA integrity (P = 0.0004). These semen characteristics were negatively affected 21 days after IN, and excluding induced TBARSs and abnormalities, recovered 35 days afterward; induced TBARSs just were affected after 49 days of IN; sperm abnormalities just recovered after 63 days. Testosterone blood serum concentration was lesser in insulated rams (P = 0.03). Thus, the difference of 1.12 degrees C between RT and testicular temperature impacts semen quality and testosterone blood serum concentration. Moreover, this study shows that rams can recover testes temperature efficiently toward IN and that infrared thermography is an efficient tool to identify differences on SSMT. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/00040-0 - Tratamento da degeneração testicular em carneiros com suplementação vitamínica e mineral ou laserterapia de baixa intensidade
• Beneficiário: Eneiva Carla Carvalho Celeghini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/15745-1 - Tratamento da degeneração testicular em carneiros com suplementação vitamínica e mineral ou laserterapia de baixa intensidade
• Beneficiário: Vinicius Silva Vellone
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 11/16744-3 - Tratamento da degeneração testicular em carneiros com suplementação vitamínica e mineral ou laserterapia de baixa intensidade
• Beneficiário: Maíra Bianchi Rodrigues Alves
• Modalidade de apoio: Bolsas no Brasil - Mestrado