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Renal Subcapsular Space of Balb/c Nude Mice as a Route for Evaluating Subpopulations of Human Bladder Carcinoma Cells

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Autor(es):
Souza, Diego Mota [1] ; Gomes Matheus, Luiz Henrique [1] ; Silva, Chrisna Souza [1] ; Ferreira, Janaina Mendes [1] ; Delle, Humberto [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Nove de Julho Univ UNINOVE, Postgrad Program Med, Rua Vergueiro 235, 2 Subsolo, BR-01504001 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo de Revisão
Fonte: IN VIVO; v. 30, n. 4, p. 383-386, JUL-AUG 2016.
Citações Web of Science: 0
Resumo

Background/Aim: Subpopulations of bladder cancer (BC) cells have been found in tumors, with different abilities for malignancy and chemotherapy resistance. The BC cell line T24 has frequently been used to evaluate this phenomenon. Since technical limits exist in orthotopic procedures, we evaluated the renal subcapsular space as an alternative route for analyzing subpopulations of T24 BC cells in vivo. Materials and Methods: Balb/c nude mice underwent renal subcapsular inoculation with T24 cells, suspended in two different volumes of PBS. Four weeks post-inoculation, histology and immunohistochemistry were carried out. Results: In all the animals inoculated with a 10 mu l volume of suspended cells, a pseudo-bladder structure in the renal subcapsular space was observed, with differential expression of mesenchymal and epithelial markers. T24 cells infiltrating the renal parenchyma towards the medulla and vessels were also observed. The volume used for inoculation was an important factor for the success of this technique. Conclusion: Renal subcapsular inoculation is an effective route for analyzing subpopulations and differentiation of T24 cells. (AU)

Processo FAPESP: 12/04423-0 - Papel da enzima indoleamina 2,3 dioxigenase (IDO) no processo de transição epitélio-mesenquimal (EMT) mediada por TGF-beta em carcinoma urogenital humano
Beneficiário:Humberto Dellê
Linha de fomento: Auxílio à Pesquisa - Regular