Excessive training impairs the insulin signal transduction in mice skeletal muscles

Autor(es)

Pereira, Bruno C. ^[1] ; da Rocha, Alisson L. ^[1] ; Pinto, Ana P. ^[1] ; Pauli, Jose R. ^[2] ; de Moura, Leandro P. ^[2] ; Mekary, Rania A. ^{[3, 4]} ; de Freitas, Ellen C. ^[5] ; da Silva, Adelino S. R. ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, BR-09500900 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Fac Sci Appl, Sport Sci Course, Sao Paulo - Brazil

^[3] Harvard Sch Publ Hlth, Dept Nutr, Boston, MA - USA

^[4] MCPHS Univ, Dept Social & Adm Sci, Boston, MA - USA

^[5] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Endocrinology; v. 230, n. 1, p. 93-104, JUL 2016.
• Citações Web of Science: 8

Resumo

The main aim of this investigation was to verify the effects of overtraining (OT) on the insulin and inflammatory signaling pathways in mice skeletal muscles. Rodents were divided into control (CT), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up), and overtrained by running without inclination (OTR) groups. Rotarod, incremental load, exhaustive, and grip force tests were used to evaluate performance. Thirty-six hours after the grip force test, the extensor digitorum longus (EDL) and soleus were extracted for subsequent protein analyses. The three OT protocols led to similar responses of all performance evaluation tests. The phosphorylation of insulin receptor beta (pIR beta; Tyr), protein kinase B (pAkt; Ser473), and the protein levels of plasma membrane glucose transporter-4 (GLUT4) were lower in the EDL and soleus after the OTR/down protocol and in the soleus after the OTR/up and OTR protocols. While the pIR beta was lower after the OTR/up and OTR protocols, the pAkt was higher after the OTR/up in the EDL. The phosphorylation of I kappa B kinase alpha and beta (pIKK alpha/beta; Ser180/181), stress-activated protein kinases/Jun amino-terminal kinases (pSAPK-JNK; Thr183/Tyr185), factor nuclear kappa B (pNF kappa B p65; Ser536), and insulin receptor substrate 1 (pIRS1; Ser307) were higher after the OTR/down protocol, but were not altered after the two other OT protocols. In summary, these data suggest that OT may lead to skeletal muscle insulin signaling pathway impairment, regardless of the predominance of eccentric contractions, although the insulin signal pathway impairment induced in OTR/up and OTR appeared to be muscle fiber-type specific. (AU)

• Processo FAPESP: 13/20591-3 - Respostas das proteínas das vias moleculares inflamatória, insulínica e hipertrófica ao overreaching não funcional induzido pelo exercício físico realizado em esteira rolante em declive, sem inclinação e em aclive em músculos esqueléticos de camundongos
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/19985-7 - Efeitos do overtraining induzido através de exercício em esteira rolante sem inclinação, em aclive e em declive, no conteúdo e ativação das proteínas das vias inflamatória e de sinalização da insulina em camundongos
• Beneficiário: Bruno Cesar Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/25459-9 - Overreaching não funcional em modelo animal: adaptações inflamatórias e hipertróficas do músculo cardíaco
• Beneficiário: Alisson Luiz da Rocha
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/08013-0 - Overreaching não funcional em modelo animal e estresse do retículo endoplasmático no músculo cardíaco e fígado
• Beneficiário: Ana Paula Pinto
• Modalidade de apoio: Bolsas no Brasil - Mestrado