L-Asparaginase Purification

Autor(es)

Tundisi, Louise L. ; Coelho, Diego F. ; Zanchetta, Beatriz ; Moriel, Patricia ; Pessoa, Jr., Adalberto ; Tambourgi, Elias B. ; Silveira, Edgar ; Mazzola, Priscila G.

• Tipo de documento: Artigo Científico
• Fonte: SEPARATION AND PURIFICATION REVIEWS; v. 46, n. 1, p. 35-43, 2016.
• Citações Web of Science: 5

Resumo

L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase. (AU)

• Processo FAPESP: 13/08617-7 - Produção de L-asparaginase extracelular: da bioprospecção à engenharia de um biofármaco antileucêmico
• Beneficiário: Adalberto Pessoa Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático