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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

L-Asparaginase Purification

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Author(s):
Tundisi, Louise L. ; Coelho, Diego F. ; Zanchetta, Beatriz ; Moriel, Patricia ; Pessoa, Jr., Adalberto ; Tambourgi, Elias B. ; Silveira, Edgar ; Mazzola, Priscila G.
Total Authors: 8
Document type: Journal article
Source: SEPARATION AND PURIFICATION REVIEWS; v. 46, n. 1, p. 35-43, 2016.
Web of Science Citations: 5
Abstract

L-asparaginase (L-ASNase) is the gold standard enzyme used to treat acute lymphoblastic leukemia. This disease primarily affects children; however, treatment increases survival from 20% to 90%. As a bioproduct, it is obtained via a biotechnological process and its purification usually consists of several steps that account for up to 80% of the total production costs. This review discusses available strategies for the purification of L-ASNase and highlights a process with fewer steps, and consequently, lower cost and higher yield. This process emphasizes the possibility of using a novel aqueous two-phase system extraction process to purify L-ASNase. (AU)

FAPESP's process: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical
Grantee:Adalberto Pessoa Junior
Support Opportunities: Research Projects - Thematic Grants