Efficient electrochemical biosensors for ethynylestradiol based on the laccase enzyme supported on single walled carbon nanotubes decorated with nanocrystalline carbon quantum dots

Autor(es)

Canevari, T. C. ; Cincotto, F. H. ; Nakamura, M. ; Machado, S. A. S. ; Toma, H. E.

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL METHODS; v. 8, n. 39, p. 7254-7259, 2016.
• Citações Web of Science: 3

Resumo

A new hybrid nanomaterial based on single-walled carbon nanotubes incorporating nanocrystalline carbon quantum dots (SWCNT/C-dots) was developed and characterized by means of high resolution transmission electron microscopy, atomic force microscopy, Raman spectroscopy and electrochemical techniques. A glassy carbon electrode modified with SWCNT/C-dots exhibited an enhanced electrocatalytic response for 17 alpha-ethynylestradiol, reflecting an increase of the active area of the hybrid material, promoted by the C-dots. However, the best activity was observed for laccase immobilized on CGE/SWCNT/C-dots, suggesting that the nanocrystalline C-dots improve the electron transport between the substrate, SWCNTs and the copper ions in the enzyme active sites. Such an association provided a very efficient bioelectrochemical sensor for the 17 alpha-ethynylestradiol endocrine interfering agent, with a detection limit of 4.0 nmol L-1 in real samples. (AU)

• Processo FAPESP: 13/24725-4 - Química supramolecular e nanotecnologia
• Beneficiário: Henrique Eisi Toma
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/18339-1 - Detecção dos interferentes endócrinos testosterona e estradiol em amostras ambientais empregando eletrodos modificados com grafeno, nanopartículas de ouro e quantum dots.
• Beneficiário: Fernando Henrique Cincotto
• Modalidade de apoio: Bolsas no Brasil - Doutorado