Cross-linked dry bonding: A new etch-and-rinse technique

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Zhou, Jianfeng ; Chiba, Ayaka ; Scheffel, Debora L. S. ; Hebling, Josimeri ; Agee, Kelli ; Tagami, Junji ; Tan, Jianquo ; Abuelenain, Dalia ; Abu Nawareg, Manar ; Hassan, Ali H. ; Breschi, Lorenzo ; Tay, Franklin R. ; Pashley, David H.

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 32, n. 9, p. 1124-1132, SEP 2016.
• Citações Web of Science: 8

Resumo

Objective. To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods. Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results. 24h microtensile bond strengths (mu TBS) in wet and dry controls were 53.5 +/- 3.6 and 9.4 +/- 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave mu TBS of 53.7 +/- 3.4 and 39.1 +/- 9.7 MPa (p <0.05), respectively, while 5% GSE in ethanol gave mu TBS of 51.2 +/- 2.3 and 35.3 +/- 2.0 MPa (p <0.05). 5% GSE in 5% EtOH/95% water gave wet and dry mu TBS of 53.0 +/- 2.3 and 55.7 +/- 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p <0.05). Significance. 5% GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/18160-7 - Infiltração de monômeros hidrófobos na dentina desmineralizada usando a técnica úmida com HEMA. Prova do conceito
• Beneficiário: Josimeri Hebling Costa
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 14/17232-4 - Infiltração de monômeros hidrófobos na dentina desmineralizada usando a técnica HEMA wet-bonding: prova de conceito
• Beneficiário: Débora Lopes Salles Scheffel
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado