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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Low-level laser therapy induces an upregulation of collagen gene expression during the initial process of bone healing: a microarray analysis

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Autor(es):
Tim, Carla Roberta ; Bossini, Paulo Sergio ; Kido, Hueliton Wilian ; Malavazi, Iran ; Kress, Marcia Regina von Zeska ; Carazzolle, Marcelo Falsarella ; Renno, Ana Claudia ; Parizotto, Nivaldo Antonio
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Journal of Biomedical Optics; v. 21, n. 8 AUG 2016.
Citações Web of Science: 4
Resumo

This study investigates the histological modifications produced by low level laser therapy (LLLT) on the first day of bone repair, as well as evaluates the LLLT effects on collagen expression on the site of a fracture. Twenty Wistar rats were distributed into a control group (CG) and a laser group (LG). Laser irradiation of Ga-AlAs laser 830 nm, 30 mW, 94 s, 2.8 J was performed in five sessions. Animals were euthanized on day 5 post-surgery. Histopathological analysis showed that LLLT was able to increase deposition of granulation tissue and newly formed bone at the site of the injury. In addition, picrosirius analysis showed that collagen fiber organization in the LG was enhanced compared to CG. Microarray analysis demonstrated that LLLT produced an upregulation type I collagen (COL-I). Immunohistochemical analysis revealed that the subjects that were treated presented a higher immunoexpression of COL-I. Our findings indicated that LLLT improves bone healing by producing a significant increase in the expression of collagen genes. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

Processo FAPESP: 10/15335-0 - Análise da expressão gênica durante o reparo ósseo em defeitos tíbias irradiados com laser terapêutico de baixa intensidade
Beneficiário:Carla Roberta Tim
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
Beneficiário:Munir Salomao Skaf
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs