Effects of photobiomodulation therapy (PBMT) on bovine sperm function

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Siqueira, Adriano F. P. ; Maria, Fernanda S. ; Mendes, Camilla M. ; Hamilton, Thais R. S. ; Dalmazzo, Andressa ; Dreyer, Thiago R. ; da Silva, Herculano M. ; Nichi, Marcilio ; Milazzotto, Marcella P. ; Visintin, Jose A. ; Assumpcao, Mayra E. O. A.

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 6, p. 1245-1250, AUG 2016.
• Citações Web of Science: 4

Resumo

Fertilization rates and subsequent embryo development rely on sperm factors related to semen quality and viability. Photobiomodulation therapy (PBMT) is based on emission of electromagnetic waves of a laser optical system that interact with cells and tissues resulting in biological effects. This interaction is mediated by photoacceptors that absorb the electromagnetic energy. Effects are dependent of irradiation parameters, target cell type, and species. In sperm, PBMT improves several features like motility and viability, affecting sperm aerobic metabolism and energy production. The aim of this study was to investigate, under same conditions, how different output powers (5, 7.5, and 10 mW) and time of irradiation (5 and 10 min) of laser (He-Ne laser, 633 nm) may affect frozen/thawed bovine sperm functions. Results showed significant effects depending on power while using 10 min of irradiation on motility parameters and mitochondrial potential. However, no effect was observed using 5 min of irradiation, regardless of power applied. In conclusion, PBMT is effective to modulate bovine sperm function. The effectiveness is dependent on the interaction between power applied and duration of irradiation, showing that these two parameters simultaneously influence sperm function. In this context, when using the same fluency and energy with different combinations of power and time of exposure, we observed distinct effects, revealing that biological effects should be also based on simple parameters rather than only composite parameters such as fluency, irradiance and energy. Laser irradiation of frozen/thawed bovine semen led to an increase on mitochondrial function and motility parameters that could potentially improve fertility rates. (AU)

• Processo FAPESP: 07/58487-1 - Influencia dos indices espermaticos de protamina sobre a fertilidade in vitro em bovinos.
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/04770-0 - Efeito do laser de baixa potência sobre a viabilidade espermática e a produção in vitro de embriões bovinos
• Beneficiário: Adriano Felipe Perez Siqueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado