Differential genotoxicity and cytotoxicity of phomoxanthone A isolated from the fungus Phomopsis longicolla in HL60 cells and peripheral blood lymphocytes

Autor(es)

Pavao, Gabriel Brolio ; Venancio, Vinicius Paula ; Leandrini de Oliveira, Ana Ligia ; Hernandes, Livia Cristina ; Almeida, Mara Ribeiro ; Greggi Antunes, Lusania Maria ; Debonsi, Hosana Maria

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 37, p. 211-217, DEC 2016.
• Citações Web of Science: 1

Resumo

Phomoxanthone A (PhoA) is a compound isolated from the endophytic fungus Phomopsis longicolla, associated with marine algae Bostrychia radicans. Although this metabolite was previously described regarding its high biological potential, there are no reports concerning the effects of this compound on DNA integrity. This study aimed to evaluate, in lymphocytes and promyelocytic leukemia HL60 cells, the cytotoxicity of this compound through MTT and neutral red (NR) assays, as well as its genotoxicity and mutagenicity by alkaline comet assay and cytokinesis-block micronucleus cytome assay (CBMN-Cyt), respectively. Cells were treated with PhoA concentrations ranging from 0.01 to 100.0 mu g/mL, and the results show that this molecule did not exhibit cytotoxicity, genotoxicity or mutagenicity in lymphocytes at any tested concentration. Furthermore, PhoA was highly cytotoxic, genotoxic and mutagenic to HL60 cells, establishing a differential response of this natural product in normal and cancer cells. PhoA was highly selective towards HL60 compared to lymphocytes, causing no damage in the latter cell line, suggesting that this compound could be a promising compound in antitumoral drug development. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/50836-2 - Estresse ambiental e modulação química epigenética: estratégias para exploração do perfil metabólico e biológico de fungos endofíticos e cianobactérias marinhas
• Beneficiário: Hosana Maria Debonsi
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular