Lamellar keratoplasty in rabbits using an allogeneic free omental graft and omentum- derived mesenchymal cells associated with the canine amniotic membrane

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da Silva Gouvea de Barros, Sefora Vieira ; Aldrovani, Marcela ; Correa de Lacerda, Luciana Cenco ; Horr, Monica ; Marinho, Fabio Andrade ; Lima, Tiago Barbalho ; Balthazar da Silveira, Camila Pinho ; da Cunha Brito, Fabio Luiz ; Bassaneze, Vinicius ; Flecher, Mayra Cunha ; Beltrame, Renato Travassos ; Nakamuta, Juliana Sanajotti ; Krieger, Jose Eduardo ; Laus, Jose Luiz

• Tipo de documento: Artigo Científico
• Fonte: Ciência Rural; v. 46, n. 10, p. 1838-1845, OCT 2016.
• Citações Web of Science: 1

Resumo

The objective of this research was to evaluate the clinical and microscopic effects in rabbits of lamellar keratoplasty using allogeneic omentum associated with canine amniotic membrane (AM). Rabbits were divided into two groups: one received the allogeneic free omental graft covered with the AM (OM-graft group), while the other received the AM graft containing omental mesenchymal cells (OM-cell group). Clinical signs were evaluated on different postoperative days. After the clinical assessments, the rabbits were euthanized and their corneas were obtained for histopathology and immunohistochemistry (Ki-67, marker for proliferation). Both groups showed chemosis, blepharospasm, eye discharge, hyperemia, and corneal opacity/edema. Neovascularization was observed in the OM-cell group. Histopathological evaluation revealed epithelial islands within the stroma of OM-cell samples. Thirty days after surgery, complete corneal re-epithelialization had occurred in both groups. The OM-cell group showed more Ki-67 positive cells. The free omentum and its cells, combined with the AM, contributed to corneal repair, a process that was completed 30 days after lamellar keratoplasty. (AU)

• Processo FAPESP: 11/17665-0 - Reparação corneal (Parte I e Parte II)
• Beneficiário: José Luiz Laus
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17308-5 - Supraorganização cromatínica, conteúdo de DNA, concentração de glicoproteínas nucleares e avaliação imunofenotípica de células epiteliais límbicas expandidas sobre membrana amniótica intacta ou desepitelizada versus membrana de fucoidam-alginato-quitosana
• Beneficiário: Marcela Aldrovani Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado