Photodynamic treatment with phenothiazinium photosensitizers kills both ungerminated and germinated microconidia of the pathogenic fungi Fusarium oxysporum, Fusarium moniliforme and Fusarium solani

Autor(es)

de Menezes, Henrique Dantas ; Tonani, Ludmilla ; Bachmann, Luciano ; Wainwright, Mark ; Leite Braga, Gilberto Ubida ; von Zeska Kress, Marcia Regina

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 164, p. 1-12, NOV 2016.
• Citações Web of Science: 6

Resumo

The search for alternatives to control microorganisms is necessary both in clinical and agricultural areas. Antimicrobial photodynamic treatment (APDT) is a promising light-based approach that can be used to control both human and plant pathogenic fungi. In the present study, we evaluated the effects of photodynamic treatment with red light and four phenothiazinium photosensitizers (PS): methylene blue (MB), toluidine blue O (TBO), new methylene blue N (NMBN) and the phenothiazinium derivative 5137 on ungerminated and germinated microconidia of Fusarium oxysporum, F. moniliforme, and F. solani. APDT with each PS killed efficiently both the quiescent ungerminated microconidia and metabolically active germinated microconidia of the three Fusarium species. Washing away the unbound PS from the microconidia (both ungerminated and germinated) before red light exposure reduced but did not prevent the effect of APDT. Subcelullar localization of PS in ungerminated and germinated microconidia and the effects of photodynamic treatment on cell membranes were also evaluated in the three Fusarium species. APDT with MB, TBO, NMBN or 5137 increased the membrane permeability in microconidia and APDT with NMBN or 5137 increased the lipids peroxidation in microconidia of the three Fusarium species. These findings expand the understanding of photodynamic inactivation of filamentous fungi with phenothiazinium PS. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/05853-7 - Efeito da inativação fotodinâmica antimicrobiana em Fonsecaea pedrosoi e em diferente espécies de Fusarium sp. utilizando fotossensibilizadores fenotiazínicos
• Beneficiário: Marcia Regina von Zeska Kress
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/15204-8 - Estudo mecanístico da inativação fotodinâmica de fungos patógenos de humanos e de fungos fitopatógenos
• Beneficiário: Gilberto Úbida Leite Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular