Bioethanol production by recycled Scheffersomyces stipitis in sequential batch fermentations with high cell density using xylose and glucose mixture

Autor(es)

Santos, Samantha Christine ; de Sousa, Amanda Silva ; Dionisio, Suzane Rodrigues ; Tramontina, Robson ; Ruller, Roberto ; Squina, Fabio Marcio ; Rossell, Carlos Eduardo Vaz ; da Costa, Aline Carvalho ; Ienczak, Jaciane Lutz

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 219, p. 319-329, NOV 2016.
• Citações Web of Science: 10

Resumo

Here, it is shown three-step investigative procedures aiming to improve pentose-rich fermentations performance, involving a simple system for elevated mass production by Scheffersomyces stipitis (I), cellular recycle batch fermentations (CRBFs) at high cell density using two temperature strategies (fixed at 30 degrees C; decreasing from 30 to 26 degrees C) (II), and a short-term adaptation action seeking to acclimatize the microorganism in xylose rich-media (III). Cellular propagation provided 0.52 g(dry) (cell) (weight) g(RS)(1), resulting in an expressive value of 45.9 g(dry cell weight) L (1). The yeast robustness in CRBF was proven by effective ethanol production, reaching high xylose consumption (81%) and EtOH productivity (1.53 g L (1) h (1)). Regarding the short-term adaptation, S. stipitis strengthened its robustness, as shown by a 6-fold increase in xylose reductase (XR) activity. The short fermentation time (20 h for each batch) and the fermentation kinetics for ethanol production from xylose are quite promising. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/01135-0 - Produção de bioetanol de segunda geração em biorreatores intensificados a partir de cepas de Scheffersomyces stipitis melhoradas por evolução induzida
• Beneficiário: Samantha Christine Santos
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/57873-8 - Um processo integrado para produção total de bioetanol e emissão zero de CO2
• Beneficiário: Rubens Maciel Filho
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático