Bioethanol production by recycled Scheffersomyces stipitis in sequential batch fermentations with high cell density using xylose and glucose mixture

Author(s)

Santos, Samantha Christine ; de Sousa, Amanda Silva ; Dionisio, Suzane Rodrigues ; Tramontina, Robson ; Ruller, Roberto ; Squina, Fabio Marcio ; Rossell, Carlos Eduardo Vaz ; da Costa, Aline Carvalho ; Ienczak, Jaciane Lutz

• Document type: Journal article
• Source: Bioresource Technology; v. 219, p. 319-329, NOV 2016.
• Web of Science Citations: 10

Abstract

Here, it is shown three-step investigative procedures aiming to improve pentose-rich fermentations performance, involving a simple system for elevated mass production by Scheffersomyces stipitis (I), cellular recycle batch fermentations (CRBFs) at high cell density using two temperature strategies (fixed at 30 degrees C; decreasing from 30 to 26 degrees C) (II), and a short-term adaptation action seeking to acclimatize the microorganism in xylose rich-media (III). Cellular propagation provided 0.52 g(dry) (cell) (weight) g(RS)(1), resulting in an expressive value of 45.9 g(dry cell weight) L (1). The yeast robustness in CRBF was proven by effective ethanol production, reaching high xylose consumption (81%) and EtOH productivity (1.53 g L (1) h (1)). Regarding the short-term adaptation, S. stipitis strengthened its robustness, as shown by a 6-fold increase in xylose reductase (XR) activity. The short fermentation time (20 h for each batch) and the fermentation kinetics for ethanol production from xylose are quite promising. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/01135-0 - Second generation bioethanol production in intensified bioreactors from improved Scheffersomyces stipitis by induced evolution
• Grantee: Samantha Christine Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission
• Grantee: Rubens Maciel Filho
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants