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Second generation bioethanol production in intensified bioreactors from improved Scheffersomyces stipitis by induced evolution

Grant number: 14/01135-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): June 01, 2014
Effective date (End): May 31, 2017
Field of knowledge:Engineering - Chemical Engineering
Principal researcher:Aline Carvalho da Costa
Grantee:Samantha Christine Santos
Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission, AP.BIOEN.TEM
Associated scholarship(s):15/14202-0 - Biological alpha-ketoglutaric acid production by Yarrowia lipolytica using lignocellulosic hydrolysate: a potential feedstock for a bio-derived compound generation, BE.EP.PD


Sugarcane bagasse (SCB) is a lignocellulosic by-product of the production of (1G) ethanol and is a promising substrate for second generation (2G) bioethanol production. Since pretreatment of a lignocellulosic biomass is required to improve bioconversion (releasing fermentable sugars such as pentoses), it also produces a great amount of chemical of inhibitors of the subsequent fermentation. Although a number of technologies have been employed to remove fermentation inhibitors from lignocellulosic hydrolysate, they increase process costs. Scheffersomyces stipitis is able to utilize pentoses by the metabolic pathway and by laboratory evolution. Induced mutagenesis techniques, such as ultraviolet (UV) radiation and chemical compounds (EMS: ethyl methane sulfonate) have been shown to lead to a significant improvement in xylose utilization by yeast cells, and thus in ethanol fermentation yields. As a preliminary step, the present work aims to evaluate S. stipitis strains tolerance, growth and bioethanol production capacity at fed batch after two distinct lignocellulosic pretreatment methods applied (using sugarcane bagasse as substrate): acid catalysis and hydrothermal. Concomitantly, sequential mutagenesis using EMS and near and far UV radiations will be conducted to obtain S. stipitis strains with genetic variability and tolerant to the inhibitor compounds at the hydrolysate. To better investigate the mutant yeast, molecular characterization of the mutant will be performed, considering differences in the genetic profile. Furthermore, a bioethanol production system with high cells density of mutant S. stipitis obtained by cell recycling will be used to examine the characteristics of bioethanol (2G) fermentation using sugarcane bagasse hydrolysate as substrate (pretreatment method selected in the preliminary step).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIAZI, LUIZ E.; SANTOS, SAMANTHA C.; NETO, ANTONIO A. KAUPERT; SOUSA, AMANDA S.; SOARES, LAUREN B.; RENZANO, EDUARDO; VELASCO, JULIANA; RABELO, SARITA C.; COSTA, ALINE C.; IENCZAK, JACIANE L.. Adaptation Strategy to Increase the Tolerance of Scheffersomyces stipitis NRRL Y-7124 to Inhibitors of Sugarcane Bagasse Hemicellulosic Hydrolysate Through Comparative Studies of Proteomics and Fermentation. BioEnergy Research, . (15/20630-4, 14/01135-0, 17/04997-0, 16/14567-0)
SANTOS, SAMANTHA CHRISTINE; DE SOUSA, AMANDA SILVA; DIONISIO, SUZANE RODRIGUES; TRAMONTINA, ROBSON; RULLER, ROBERTO; SQUINA, FABIO MARCIO; ROSSELL, CARLOS EDUARDO VAZ; DA COSTA, ALINE CARVALHO; IENCZAK, JACIANE LUTZ. Bioethanol production by recycled Scheffersomyces stipitis in sequential batch fermentations with high cell density using xylose and glucose mixture. Bioresource Technology, v. 219, p. 319-329, . (14/01135-0, 08/57873-8)

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