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Scheffersomyces stipitis and Spathaspora passalidarum wild type yeast assessment for second generation bioethanol production from bagasse sugarcarne hydrolyzates containing xylose and glucose

Grant number: 17/04997-0
Support type:Regular Research Grants
Duration: August 01, 2017 - July 31, 2019
Field of knowledge:Engineering - Chemical Engineering - Chemical Process Industries
Principal Investigator:Jaciane Lutz Ienczak
Grantee:Jaciane Lutz Ienczak
Home Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil
Assoc. researchers:Aline Carvalho da Costa ; Gabriela Felix Persinoti ; Leandro Vieira dos Santos ; Sarita Candida Rabelo


The main features of the first generation ethanol production Brazilian Mills are the use of high yeast density, reuse of yeasts (economy of sugar due to the low cell growth), fed batch (avoid inhibition by substrate), suitable temperature and pH, microbial control through application of acid washes between recycles that enables Melle Boinot process. For another hand, the fermentation of sugars obtained by cane sugar biomass hydrolysis (glucose and xylose) is still a process in development. In this sense, fermentation of these sugars by native yeasts and the use of the success system used on first generation (Melle Boinot) are shown as the starting point for the establishment of a process safe for the second generation ethanol production, requiring some adaptations to the new reality of the process (yeast non Saccharomyces cerevisiae and different carbohydrates). In this sense, this project aim at studying the adequacy of Melle Boinot process variables for fermentation of sugarcane bagasse hydrolysates that contains mixture of xylose and glucose by native yeasts Scheffersomyces stipitis and Spathaspora passalidarum. The project goal to study the following points: best temperature for xylose consumption by these yeasts, modulation of xylose and glucose metabolism for these yeasts, influence of oxygen transfer in the process, fermentation vats contamination, mathematical models that describe the behavior of these yeasts, kinetic and system mass balance. By the results obtained with this project will be possible to understand xylose and glucose consumption for ethanol production by wild type yeast at biochemical / biological level (analysis of metabolites and transcripts), bioprocesses level (mass transfer, inhibitors and contaminants effect) and technological application (application in pilot scale) of the process based on the first generation (Melle Boinot). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BIAZI, L. E.; MARTINEZ-JIMENEZ, F. D.; BONAN, C. I. D. G.; SOARES, L. B.; MORAIS, E. R.; IENCZAK, J. L.; COSTA, A. C. A differential evolution approach to estimate parameters in a temperature-dependent kinetic model for second generation ethanol production under high cell density with Spathaspora passalidarum. Biochemical Engineering Journal, v. 161, SEP 15 2020. Web of Science Citations: 0.
BONAN, CAROLINA I. D. G.; BIAZI, LUIZ E.; DIONISIO, SUZANE R.; SOARES, LAUREN B.; TRAMONTINA, ROBSON; SOUSA, AMANDA S.; DE OLIVEIRA FILHO, CARLOS A.; COSTA, ALINE C.; IENCZAK, JACIANE L. Redox potential as a key parameter for monitoring and optimization of xylose fermentation with yeast Spathaspora passalidarum under limited-oxygen conditions. Bioprocess and Biosystems Engineering, v. 43, n. 8 APR 2020. Web of Science Citations: 0.
COLLOGRAI, KAREN CRISTINA; DA COSTA, ALINE CARVALHO; IENCZAK, JACIANE LUTZ. Effect of contamination with Lactobacillus fermentum I2 on ethanol production by Spathaspora passalidarum. Applied Microbiology and Biotechnology, v. 103, n. 12, p. 5039-5050, JUN 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
IENCZAK, Jaciane Lutz. . 2019. Master's Dissertation.

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