| Texto completo | |
| Autor(es): |
Garcia-Neto, Wilson
;
Cabrera-Orefice, Alfredo
;
Uribe-Carvajal, Salvador
;
Kowaltowski, Alicia J.
;
Luevano-Martinez, Luis Alberto
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 12, n. 1 JAN 6 2017. |
| Citações Web of Science: | 4 |
| Resumo | |
The oleaginous yeast Debaryomyces hansenii is a good model to understand molecular mechanisms involved in halotolerance because of its impressive ability to survive under a wide range of salt concentrations. Several cellular adaptations are implicated in this response, including the presence of a cyanide-insensitive ubiquinol oxidase (Aox). This protein, which is present in several taxonomical orders, has been related to different stress responses. However, little is known about its role in mitochondria during transitions from low to high saline environments. In this report, we analyze the effects of Aox in shifts from low to high salt concentrations in the culture media. At early stages of a salt insult, we observed that this protein prevents the overflow of electrons on the mitochondrial respiratory chain, thus, decreasing the production of reactive oxygen species. Interestingly, in the presence of high osmolite concentrations, Aox activity is able to sustain a stable membrane potential when coupled to complex I, despite a compromised cytochrome pathway. Taken together, our results suggest that under high osmolarity conditions Aox plays a critical role regulating mitochondrial physiology. (AU) | |
| Processo FAPESP: | 13/04919-9 - Avaliação da resposta adaptativa das membranas mitocondriais em situações de estresse: papel da cardiolipina na plasticidade da membrana, dinâmica e situações patológicas |
| Beneficiário: | Luis Alberto Luevano Martinez |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |