| Texto completo | |
| Autor(es): Mostrar menos - |
Hamley, Ian W.
;
Burholt, Samuel
;
Hutchinson, Jessica
;
Castelletto, Valeria
;
da Silva, Emerson Rodrigo
;
Alves, Wendel
;
Gutfreund, Philipp
;
Porcar, Lionel
;
Dattani, Rajeev
;
Hermida-Merino, Daniel
;
Newby, Gemma
;
Reza, Mehedi
;
Ruokolainen, Janne
;
Stasiak, Joanna
Número total de Autores: 14
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biomacromolecules; v. 18, n. 1, p. 141-149, JAN 2017. |
| Citações Web of Science: | 8 |
| Resumo | |
The bola-amphiphilic arginine-capped peptide RFL4RF self-assembles into nanotubes in aqueous solution. The nanostructure and rheology are probed by in situ simultaneous rheology/small-angle scattering experiments including rheo-SAXS, rheo-SANS, and rheo-GISANS (SAXS: small-angle X-ray scattering, SANS: small-angle neutron scattering, GISANS: grazing incidence small-angle neutron scattering). Nematic alignment of peptide nanotubes under shear is observed at sufficiently high shear rates under steady shear in either Couette or cone-and-plate geometry. The extent of alignment increases with shear rate. A shear plateau is observed in a flow curve measured in the Couette geometry, indicating the presence of shear banding above the shear rate at which significant orientation is observed (0.1-1 s(-1)). The orientation under shear is transient and is lost as soon as shear is stopped. GISANS shows that alignment at the surface of a cone-and-plate cell develops at sufficiently high shear rates, very similar to that observed in the bulk using the Couette geometry. A small isotope effect (comparing H2O/D2O solvents) is noted in the CD spectra indicating increased interpeptide hydrogen bonding in D2O, although this does not influence nanotube self-assembly. These results provide new insights into the controlled alignment of peptide nanotubes for future applications. (AU) | |
| Processo FAPESP: | 13/12674-6 - Investigações estruturais em sistemas-modelo à base de peptídeos anfifílicos |
| Beneficiário: | Emerson Rodrigo da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção |
| Beneficiário: | Wendel Andrade Alves |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |