Bioactive glass-based surfaces induce differential gene expression profiling of osteoblasts

Autor(es)

Ferraz, Emanuela P. ^[1] ; Oliveira, Fabiola S. ^[1] ; de Oliveira, Paulo T. ^[1] ; Crovace, Murilo C. ; Peitl-Filho, Oscar ^[2] ; Beloti, Marcio M. ^[1] ; Rosa, Adalberto L.

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Vitreous Mat Lab LaMaV, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Rosa, Adalberto L., Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP, Brazil. Crovace, Murilo C., Univ Fed Sao Carlos, Vitreous Mat Lab LaMaV, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 105, n. 2, p. 419-423, FEB 2017.
• Citações Web of Science: 7

Resumo

The ability of Biosilicate (R) with two crystalline phases (BioS-2P) to drive osteoblast differentiation encourages the investigation of the cellular mechanisms involved in this process. Then, the aim of our study was to analyze the large-scale gene expression of osteoblasts grown on BioS-2P compared with Bioglass((R))45S5 (45S5). Osteoblasts differentiated from rat bone marrow mesenchymal stem cells were cultured under osteogenic conditions on BioS-2P, 45S5 and polystyrene (control). After 10 days, the expression of 23,794 genes was analyzed using mRNA Sequencing and the data were validated by real-time PCR. The BioS-2P exhibited 5 genes upregulated and 3 downregulated compared with 45S5. Compared with control, BioS-2P upregulated 15 and downregulated 11 genes, while 45S5 upregulated 25 and downregulated 21 genes. Eight genes were commonly upregulated and 4 downregulated by both bioactive glasses. In conclusion, our results demonstrated that bioactive glasses affect the gene expression profiling of osteoblasts. Most of the regulated genes by both BioS-2P and 45S5 are associated with the process of mineralization highlighting their osteostimulation property that is, at least in part, derived from the ability to modulate the intracellular machinery to promote osteoblast genotype expression. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 419-423, 2017. (AU)

• Processo FAPESP: 12/23879-5 - Engenharia de tecidos: efeito da associação entre células e Bio-sca sobre o reparo de defeitos ósseos
• Beneficiário: Adalberto Luiz Rosa
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/03036-9 - Análise da expressão em larga escala de microRNAs nas amostras de RNA obtidas de culturas de células osteoblásticas cultivadas sobre biosilicato e Bio-sca
• Beneficiário: Emanuela Prado Ferraz
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 12/23525-9 - Engenharia de tecidos: efeito da associação entre células e Bio-sca sobre o reparo de defeitos ósseo
• Beneficiário: Emanuela Prado Ferraz
• Linha de fomento: Bolsas no Brasil - Doutorado