Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil

Autor(es)

Dutra, Karina Rocha ; Drumond, Betania Paiva ; de Rezende, Izabela Mauricio ; Nogueira, Mauricio Lacerda ; Lopes, Debora de Oliveira ; Calzavara Silva, Carlos Eduardo ; Siqueira Ferreira, Jaqueline Maria ; dos Santos, Luciana Lara

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 89, n. 6, p. 966-973, JUN 2016.
• Citações Web of Science: 0

Resumo

Dengue, caused by any of the four types of Dengue virus (DENV) is the most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinopolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with clinical symptoms of dengue were studied and 26 were positive. The capsid/premembrane (CprM) and envelope gene sequences of some samples were amplified and sequenced. Molecular analyses demonstrated that two DENV-1 lineages, belonging to genotype V were introduced and co-circulated in Divinopolis. When compared to each other, those lineages presented high genetic diversity and showed unique amino acids substitutions in the envelope protein, including in domains I, II, and III. DENV-4 strains from Divinopolis clustered within genotype IIb and the most recent common ancestor was probably introduced into the city three years before the 2013 epidemic. Here we demonstrated for the first time the circulation of DENV-4 and the co-circulation of two DENV-1 lineages in Midwest region of Minas Gerais, Brazil. Moreover our analysis indicated the introduction of five DENV-1 lineages, genotype V into Brazil, in different times. J. Med. Virol. 89:966-973, 2017. (c) 2016 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Linha de fomento: Auxílio à Pesquisa - Temático