| Texto completo | |
| Autor(es): |
Silva-Alves, Luana Maria
;
Gama De Barcelos Filho, Procopio Cleber
;
Franci, Celso Rodrigues
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Neuroscience; v. 349, p. 239-252, MAY 4 2017. |
| Citações Web of Science: | 2 |
| Resumo | |
The sympato-adrenal-system and hypothalamus pituitary-adrenal (HPA) axis are anatomically and functionally connected with participation of several brain areas that express estrogen receptors (ER alpha and ER beta). We assessed the neuronal activity of these areas for FOS expression and the action of PPT (ER alpha agonist) or DPN (ER beta agonist) in HPA axis activity during hemorrhagic stress. Ovariectomized Wistar rats treated with vehicle (DMSO) or ER agonists were catheterized for blood collection. Animals received (control) or not (hemorrhagic) immediate reposition with the same volume of saline. lmmunohistochemistry was performed for FOS, tyrosine hydroxylase (TH) and corticotropin releasing hormone (CRH) in the brain areas. In vehicle-treated animals, hemorrhage enhanced: plasma corticosterone (CORT), oxytocin (OT) and vasopressin (AVP) measured by radioimmunoassay; the expression of TH-FOS co-localized neurons in ventrolateral medulla (A1C1) and FOS expression in medial parvocellular paraventricular nucleus (mpPVN). In controls, PPT decreased: plasma CORT; FOS expression at locus coeruleus (LC); FOS and CRH-FOS at mpPVN, compared to vehicle. After hemorrhage, PPT decreased: plasma CORT; FOS expression at LC and mpPVN; TH-FOS at LC, solitary tract nucleus (NTS), A1C1; CRH-FOS at mpPVN, compared to vehicle. After hemorrhage DPN decreased: plasma CORT; FOS expression at LC and mpPVN; TH-FOS at LC, A1C1; CRH-FOS at mpPVN, compared to vehicle. PPT blocked the increase of OT secretion and increased AVP secretion, after hemorrhage. DPN reduced OT and increased AVP levels, regardless hemorrhage. In hemorrhagic stress, ER alpha and ER beta reduced the HPA axis activation and neuronal activity in brain areas involved in the HPA axis control. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 12/50073-1 - Atividade do eixo hipotalamo hipofise adrenal no estresse hemorragico: possivel interacao estrogeno-ocitocina e participacao diferenciada de receptores tipos alfa e beta para estrogeno. |
| Beneficiário: | Celso Rodrigues Franci |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |