Structural features of PhoX, one of the phosphate-binding proteins from Pho regulon of Xanthomonas citri

Autor(es)

Pegos, Vanessa R. ; Santos, Rodrigo M. L. ; Medrano, Francisco J. ; Balan, Andrea

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 5 MAY 22 2017.
• Citações Web of Science: 0

Resumo

In Escherichia coli, the ATP-Binding Cassette transporter for phosphate is encoded by the pstSCAB operon. PstS is the periplasmic component responsible for affinity and specificity of the system and has also been related to a regulatory role and chemotaxis during depletion of phosphate. Xanthomonas citri has two phosphate-binding proteins: PstS and PhoX, which are differentially expressed under phosphate limitation. In this work, we focused on PhoX characterization and comparison with PstS. The PhoX three-dimensional structure was solved in a closed conformation with a phosphate engulfed in the binding site pocket between two domains. Comparison between PhoX and PstS revealed that they originated from gene duplication, but despite their similarities they show significant differences in the region that interacts with the permeases. (AU)

• Processo FAPESP: 13/09172-9 - Caracterização estrutural e funcional do transportador do tipo ABC e enzimas associadas envolvidos com a captação de sulfato em Xanthomonas citri
• Beneficiário: Andrea Balan Fernandes
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/20468-1 - Modelos biológicos de interação planta-patógeno para o entendimento de mecanismos de patogenicidade e adaptação de fitobactérias, respostas de defesa e desenvolvimento de doença em citros
• Beneficiário: Celso Eduardo Benedetti
• Linha de fomento: Auxílio à Pesquisa - Temático