Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1

Texto completo
Autor(es):
Johns, M. A. ; Bernardes, A. ; Ribeiro De Azevedo, E. ; Guimaraes, F. E. G. ; Lowe, J. P. ; Gale, E. M. ; Polikarpov, I. ; Scott, J. L. ; Sharma, R. I.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MATERIALS CHEMISTRY B; v. 5, n. 21, p. 3879-3887, JUN 7 2017.
Citações Web of Science: 8
Resumo

Cellulose-based hydrogel materials prepared by regeneration from cellulose solutions in ionic liquids, or ionic liquid containing solvent mixtures (organic electrolyte solutions), are becoming widely used in a range of applications from tissue scaffolds to membrane ionic diodes. In all such applications knowledge of the nature of the hydrogel with regards to porosity (pore size and tortuosity) and material structure and surface properties (crystallinity and hydrophobicity) is critical. Here we report significant changes in hydrogel properties, based on the choice of cellulose raw material (alpha- or bacterial cellulose - with differing degree of polymerization) and regeneration solvent (methanol or water). Focus is on bioaffinity applications, but the findings have wide ramifications, including in biomedical applications and cellulose saccharification. Specifically, we report that the choice of cellulose and regeneration solvent influences the surface area accessible to a family 1 carbohydrate-binding module (CBM), CBM affinity for the cellulose material, and rate of migration through the hydrogel. By regenerating bacterial cellulose in water, a maximum accessible surface area of 33 m(2) g(-1) was achieved. However, the highest CBM migration rate, 1.76 mu m(2) min(-1), was attained by regenerating alpha-cellulose in methanol, which also resulted in the maximum affinity of the biomolecule for the material. Thus, it is clear that if regenerated cellulose hydrogels are to be used as support materials in bioaffinity (or other) applications, a balance between accessible surface area and affinity, or migration rate, must be achieved. (AU)

Processo FAPESP: 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático