Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes

Autor(es)

Gomes, Eriston V. ; Ulhoa, Cirano J. ; Cardoza, Rosa E. ; Silva, Roberto N. ; Gutierrez, Santiago

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 8, MAY 29 2017.
• Citações Web of Science: 6

Resumo

Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichoderma harzianum Epl-1 protein during the interactions of mutant triangle epl-1 or wild-type T. harzianum strains with: (a) the phytopathogen Botrytis cinerea and (b) with tomato plants, on short (24 h hydroponic cultures) and long periods (4-weeks old plants) after Trichoderma inoculation. Our results indicate that T. harzianum Epl-1 protein affects the in vitro expression of B. cinerea virulence genes, especially those involved in the botrydial biosynthesis (BcBOT genes), during the mycoparasitism interaction. The tomato defense-related genes were also affected, indicating that Epl-1 is involved in the elicitation of the salicylic acid pathway. Moreover, Epl-1 also regulates the priming effect in host tomato plants and contributes to enhance the interaction with the host tomato plant during the early stage of root colonization. (AU)

• Processo FAPESP: 13/24452-8 - O papel da proteína elicitora de Trichoderma harzianum (Epl1) na via metabólica secundária de terpenos em resposta a infecção por Sclerotinia sclerotiorum e resposta de resistência em plantas
• Beneficiário: Eriston Vieira Gomes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 12/16895-4 - Estudo de genes de Trichoderma harzianum associados ao micoparasitismo e seu papel no biocontrole de Sclerotinia sclerotiorum
• Beneficiário: Eriston Vieira Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/04274-6 - Fatores de transcrição e sinalização celular
• Beneficiário: Eriston Vieira Gomes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado