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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Carbonic anhydrase activation enhances object recognition memory in mice through phosphorylation of the extracellular signal-regulated kinase in the cortex and the hippocampus

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Autor(es):
de Souza, Lucas Canto ; Provensi, Gustavo ; Vullo, Daniela ; Carta, Fabrizio ; Scozzafava, Andrea ; Costa, Alessia ; Schmidt, Scheila Daiane ; Passani, Maria Beatrice ; Supuran, Claudiu T. ; Blandina, Patrizio
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: Neuropharmacology; v. 118, p. 148-156, MAY 15 2017.
Citações Web of Science: 30
Resumo

Rats injected with by D-phenylalanine, a carbonic anhydrase (CA) activator, enhanced spatial learning, whereas rats given acetazolamide, a CA inhibitor, exhibited impairments of fear memory consolidation. However, the related mechanisms are unclear. We investigated if CAs are involved in a non-spatial recognition memory task assessed using the object recognition test (ORT). Systemic administration of acetazolamide to male CD1 mice caused amnesia in the ORT and reduced CA activity in brain homogenates, while treatment with D-phenylalanine enhanced memory and increased CA activity. We provided also the first evidence that D-phenylalanine administration rapidly activated extracellular signal regulated kinase (ERK) pathways, a critical step for memory formation, in the cortex and the hippo campus, two brain areas involved in memory processing. Effects elicited by D-phenylalanine were completely blunted by co-administration of acetazolamide, but not of 1-N-(4-sulfamoylphenyl-ethyl)2,4,6-trimethylpyridinium perchlorate ((C18).) a CA inhibitor that, differently from acetazolamide, does not cross the blood brain barrier. Our results strongly suggest that brain but not peripheral CAs activation potentiates memory as a result of ERK pathway enhanced activation. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 12/05838-0 - Avaliação da interação entre a neurotransmissão histaminérgica e colinérgica sobre a memória de reconhecimeto de objetos e emocional
Beneficiário:Lucas Canto de Souza
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado