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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Sulfate-Binding Protein (Sbp) from Xanthomonas citri: Structure and Functional Insights

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Autor(es):
Pereira, Cristiane Tambascia ; Roesler, Cassia ; Faria, Jessica Nascimento ; Fessel, Melissa Regina ; Balan, Andrea
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 30, n. 7, p. 578-588, JUL 2017.
Citações Web of Science: 3
Resumo

The uptake and transport of sulfate in bacteria is mediated by an ATP-binding cassette transporter (ABC transporter) encoded by sbpcysUWA genes, whose importance has been widely demonstrated due to their relevance in cysteine synthesis and bacterial growth. In Xanthomonas citri, the causative agent of canker disease, the expression of components from this ABC transporter and others related to uptake of organic sulfur sources has been shown during in vitro growth cultures. In this work, based on gene reporter and proteomics analyses, we showed the activation of the promoter that controls the sbpcysUWA operon in vitro and in vivo and the expression of sulfate-binding protein (Sbp), a periplasmic-binding protein, indicating that this protein plays an important function during growth and that the transport system is active during Citrus sinensis infection. To characterize Sbp, we solved its three-dimensional structure bound to sulfate at 1.14 angstrom resolution and performed biochemical and functional characterization. The results revealed that Sbp interacts with sulfate without structural changes, but the interaction induces a significant increasing of protein thermal stability. Altogether, the results presented in this study show the evidence of the functionality of the ABC transporter for sulfate in X. citri and its relevance during infection. (AU)

Processo FAPESP: 13/16094-4 - Estudos funcionais e estruturais sobre o transportador do tipo ABC de sulfato em Xanthomonas citri
Beneficiário:Cristiane Tambascia Pereira
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/09172-9 - Caracterização estrutural e funcional do transportador do tipo ABC e enzimas associadas envolvidos com a captação de sulfato em Xanthomonas citri
Beneficiário:Andrea Balan Fernandes
Linha de fomento: Auxílio à Pesquisa - Regular