DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Autor(es)

Margraf-Ferreira, A. ; Carvalho, I. C. S. ; Machado, S. M. ; Pacheco-Soares, C. ; Galvao, C. W. ; Etto, R. M. ; da Silva, N. S.

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 18, p. 193-197, JUN 2017.
• Citações Web of Science: 0

Resumo

Photodynamic therapy (PDT) is a modality of therapy that involves the activation of photosensitive substances and the generation of cytotoxic oxygen species and free radicals to promote the selective destruction of target tissues. This study analyzed the application of PDT to Tritrichomonas foetus, a scourged and etiological agent of bovine trichomoniasis, a sexually transmitted infectious disease. As it is an amitochondrial and aerotolerant protozoan, it produces energy under low 02 tension via hydrogenosome. T. foetus from an axenic culture was incubated with photosensitizer tetrasulfonated aluminium phthalocyanine and then irradiated with a laser source (InGaAIP) at a density of 4.5 J cm(-2). The DNA integrity of the control and treated group parasites was analyzed by conventional gel electrophoresis and comet assay techniques. In previous results, morphological changes characterized by apoptotic cell death were observed after T. foetus was submitted to PDT treatment. In the treated groups, T. foetus DNA showed a higher concentration of small fragments, about 200 pb, in gel electrophoresis after PDT. In the comet assay, the DNA tail percentage was significantly higher in the treated groups. These results demonstrate that PDT leads to DNA fragmentation with changes in nuclear morphology and apoptotic features. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/20054-8 - Estudos de adesão, proliferação e de morte celular na interação de células com materiais nanoestruturados
• Beneficiário: Newton Soares da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular