Pollination and in vitro germination of seeds for interspecific hybridization of Psidium guajava and Psidium cattleianum

Autor(es)

Cardoso, Jean Carlos ; Costa, Bruna Teixeira ; de Almeida, Eduardo Jose

• Tipo de documento: Artigo Científico
• Fonte: EUPHYTICA; v. 213, n. 7 JUL 2017.
• Citações Web of Science: 0

Resumo

The genus Psidium includes important fruit crops. However, there are very few studies focusing on its reproductive biology, which limits the establishment of breeding programs. The present work investigated the reproductive biology of Psidium guajava and Psidium cattleianum in terms of compatibility of crossings between these two species aiming at interspecific hybridization because the latter species is an important source of resistance against the nematode Meloidogyne enterolobii. Several types of crosses were performed to understand the reproductive biology of these species, including the compatibility of intra-and interspecific crossings, using assisted in vivo germination of pollen grains on the stigma. In addition, the in vitro germination of both Psidium species was studied at different stages of fruit development to rescue young seeds to improve the chances of obtaining the hybrids. No fruits of 270 pollinations were obtained on guava buds at the pre-anthesis stage, regardless of the source of the pollen grain and the cultivar used as female genotype. Microscopic analyzes demonstrated the germination of pollen grains and pollen tube growth at crosses between guava cv. `Pedro Sato' (P. guajava) and Psidium cattleianum. High germination percentages of Psidium cattleianum seeds were obtained in MS medium without sucrose or containing 15 g/L of this carbohydrate. (AU)

• Processo FAPESP: 13/17372-8 - Obtenção de plantas autotetraplóides de goiabeira (Psidium guajava)
• Beneficiário: Bruna Teixeira Costa
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/07094-0 - Utilização de ferramentas biotecnológicas em goiabeira (Psidium guajava L.) visando a resistência a Meloidogyne enterolobii
• Beneficiário: Jean Carlos Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular