Host range, mating type and population structure of Magnaporthe sp of a single barley field in Sao Paulo state, Brazil

Autor(es)

Urashima, Alfredo Seiiti ; Alves, Augusto Freitas ; Silva, Flavio Nunes ; Oliveira, Danielle ; Gazaffi, Rodrigo

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYTOPATHOLOGY; v. 165, n. 7-8, p. 414-424, AUG 2017.
• Citações Web of Science: 2

Resumo

Brazil is blast disease hot spot because severe epidemics have occurred among wheat, triticale, rye, barley and oat crops. Although the first outbreak of barley blast appeared in 1998, little information is available. Therefore, this study aimed to examine host range, mating type composition and population structure of Magnaporthe sp. from a single barley field in SAo Paulo, Brazil. To examine pathogenicity, 25 Magnaporthe isolates were inoculated on five, three, two and two cultivars of barley, wheat, oat and rice, respectively, and one cultivar each of rye, corn, sorghum, triticale and certain weeds (Cenchrus echinatus, Setaria geniculata, Brachiaria plantaginea and Eleusine indica). Mating type distribution of 33 isolates was investigated by molecular tools. The genotypic divergence of 41 barley and five wheat isolates was investigated by 15 random amplified polymorphic DNA primers and unweighted pair group method with arithmetic mean. The host range of the barley blast pathogen included wheat, oat, rye and triticale but not rice and weeds. Sexual reproduction appeared to not be involved in the high genotypic diversity because only a single isolate, MAT1-2, was identified. The majority of barley isolates clustered together with wheat blast, except for four, suggesting a different origin. (AU)

• Processo FAPESP: 13/17229-0 - Caracterização patogênica e molecular de Magnaporthe oryzae, causador da brusone em cevada
• Beneficiário: Augusto de Freitas Alves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica