The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70S6K1 and AMPK signaling pathway responses in rat skeletal muscle

Autor(es)

E.O.de Souza ^[1] ; V. Tricoli ^[2] ; C. Bueno Junior ^[3] ; M.G. Pereira ^[4] ; P.C. Brum ^[5] ; E.M. Oliveira ^[6] ; H. Roschel ^[7] ; M.S. Aoki ^[8] ; C. Urginowitsch ^[9]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

^[2] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

^[3] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte de Ribeirão Preto - Brasil

^[4] Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Anatomia - Brasil

^[5] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

^[6] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

^[7] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

^[8] Universidade de São Paulo. Escola de Artes, Ciências e Humanidades - Brasil

^[9] Universidade de São Paulo. Escola de Educaç;ão Física e Esporte - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 46, n. 4, p. 343-347, 2013-04-19.

Resumo

The activation of competing intracellular pathways has been proposed to explain the reduced training adaptations after concurrent strength and endurance exercises (CE). The present study investigated the acute effects of CE, strength exercises (SE), and endurance exercises (EE) on phosphorylated/total ratios of selected AMPK and Akt/mTOR/p70S6K1 pathway proteins in rats. Six animals per exercise group were killed immediately (0 h) and 2 h after each exercise mode. In addition, 6 animals in a non-exercised condition (NE) were killed on the same day and under the same conditions. The levels of AMPK, phospho-Thr172AMPK (p-AMPK), Akt, phospho-Ser473Akt (p-Akt), p70S6K1, phospho-Thr389-p70S6K1(p-p70S6K1), mTOR, phospho-Ser2448mTOR (p-mTOR), and phospho-Thr1462-TSC2 (p-TSC2) expression were evaluated by immunoblotting in total plantaris muscle extracts. The only significant difference detected was an increase (i.e., 87%) in Akt phosphorylated/total ratio in the CE group 2 h after exercise compared to the NE group (P = 0.002). There were no changes in AMPK, TSC2, mTOR, or p70S6K1 ratios when the exercise modes were compared to the NE condition (P ≥ 0.05). In conclusion, our data suggest that low-intensity and low-volume CE might not blunt the training-induced adaptations, since it did not activate competing intracellular pathways in an acute bout of strength and endurance exercises in rat skeletal muscle. (AU)

• Processo FAPESP: 10/51428-2 - Efeitos do treinamento de força associado à oclusão vascular em pacientes com osteoartrite de joelho: um estudo clínico controlado
• Beneficiário: Hamilton Augusto Roschel da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/02738-6 - Efeito do treinamento concorrente na expressão gênica associada à hipertrofia muscular
• Beneficiário: Eduardo Oliveira de Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado