| Texto completo | |
| Autor(es): |
Rodrigues, L. F.
;
Ierich, J. C. M.
;
Andrade, M. A.
;
Hausen, M. A.
;
Leite, F. L.
;
Moreau, Alberto L. D.
;
Steffens, Clarice
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NANO; v. 12, n. 7 JUL 2017. |
| Citações Web of Science: | 3 |
| Resumo | |
Nanomechanical biosensors based on atomic force microscopy (AFM) cantilevers have garnered considerable attention. AFM cantilevers are devices that can detect a target either via a surface functionalization process based on immobilization through molecular adsorption, or through the selective chemical binding of a specific molecule, transforming the device into a specific biosensor. In this study, we demonstrate that functionalized AFM cantilevers could be used, in a process involving self-assembling layers, to create a homogeneous surface layer of the widely used herbicide mesotrione. Controlled experiments to evaluate its detection were performed, and binding between mesotrione and its target molecule, 4-hydroxyphenylpyruvate dioxygenase (HPPD), was evaluated using deflection curves of functionalized cantilevers interacting with mesotrione. The cantilevers worked as nanomechanical sensors inside a fluid cell device, under different concentrations of HPPD diluted in PBS. After evaluating increasing concentrations of HPPD, the deflection curves showed a clear, dose-dependent pattern. The homogeneous dispersion of mesotrione on the cantilevers was assessed by confocal microscopy, and this corroborated the functionalization method. Thus, the results obtained by this functionalized cantilever presented a high efficiency in detecting binding between HPPD and mesotrione molecules at concentrations as low as 17 ng mL(-1). In this way, as a preliminary step for a future environmental contaminants nanosensor development, the described detection method showed a suitable capability for molecular recognition at the nanoscale. (AU) | |
| Processo FAPESP: | 14/12082-4 - Modelagem molecular das interações do complexo antígeno-anticorpo na investigação de doenças desmielinizantes autoimunes |
| Beneficiário: | Jéssica Cristiane Magalhães Ierich |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |