Bioactive-glass ceramic with two crystalline phases (BioS-2P) for bone tissue engineering

Autor(es)

Ferraz, Emanuela Prado ; Freitas, Gileade Pereira ; Crovace, Murilo Camuri ; Peitl, Oscar ; Zanotto, Edgar Dutra ; de Oliveira, Paulo Tambasco ; Beloti, Marcio Mateus ; Rosa, Adalberto Luiz

• Tipo de documento: Artigo Científico
• Fonte: Biomedical Materials; v. 12, n. 4 AUG 25 2017.
• Citações Web of Science: 4

Resumo

We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded with MSCs. UMR-106 proliferation was similar for both materials, while alkaline phosphatase (ALP) activity and mineralization were higher for BioS-2P. Bone sialoprotein (BSP), RUNX2 and osteopontin (OPN) gene expression and BSP, OPN, ALP and RUNX2 protein expression were higher on BioS-2P. For MSCs, ALP activity was higher on Bioglass 45S5 than on BioS-2P and was lower on polystyrene. All genes were highly expressed on bioactive glasses compared to polystyrene. BioS-2P scaffolds promoted in vivo bone formation without differences in the morphometric parameters at 4, 8 and 12 weeks. After 8 weeks, the combination of BioS-2P with MSCs did not increase the quantity of new bone compared to the BioS-2P alone. To stimulate osteoblast activity, drive MSC differentiation and promote bone formation, BioS-2P is a good choice as a scaffold for bone tissue engineering. (AU)

• Processo FAPESP: 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros
• Beneficiário: Edgar Dutra Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/23879-5 - Engenharia de tecidos: efeito da associação entre células e Bio-sca sobre o reparo de defeitos ósseos
• Beneficiário: Adalberto Luiz Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/23525-9 - Engenharia de tecidos: efeito da associação entre células e bio-sca sobre o reparo de defeitos ósseo
• Beneficiário: Emanuela Prado Ferraz
• Modalidade de apoio: Bolsas no Brasil - Doutorado