| Texto completo | |
| Autor(es): |
Ferraz, Emanuela Prado
;
Freitas, Gileade Pereira
;
Crovace, Murilo Camuri
;
Peitl, Oscar
;
Zanotto, Edgar Dutra
;
de Oliveira, Paulo Tambasco
;
Beloti, Marcio Mateus
;
Rosa, Adalberto Luiz
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biomedical Materials; v. 12, n. 4 AUG 25 2017. |
| Citações Web of Science: | 4 |
| Resumo | |
We aimed to evaluate the in vitro osteogenic and osteoinductive potentials of BioS-2P and its ability to promote in vivo bone repair. To investigate osteogenic potential, UMR-106 osteoblastic cells were cultured on BioS-2P and Bioglass 45S5 discs in osteogenic medium. The osteoinductive potential was evaluated using mesenchymal stem cells (MSCs) cultured on BioS-2P, Bioglass 45S5 and polystyrene in non-osteogenic medium. Rat bone calvarial defects were implanted with BioS-2P scaffolds alone or seeded with MSCs. UMR-106 proliferation was similar for both materials, while alkaline phosphatase (ALP) activity and mineralization were higher for BioS-2P. Bone sialoprotein (BSP), RUNX2 and osteopontin (OPN) gene expression and BSP, OPN, ALP and RUNX2 protein expression were higher on BioS-2P. For MSCs, ALP activity was higher on Bioglass 45S5 than on BioS-2P and was lower on polystyrene. All genes were highly expressed on bioactive glasses compared to polystyrene. BioS-2P scaffolds promoted in vivo bone formation without differences in the morphometric parameters at 4, 8 and 12 weeks. After 8 weeks, the combination of BioS-2P with MSCs did not increase the quantity of new bone compared to the BioS-2P alone. To stimulate osteoblast activity, drive MSC differentiation and promote bone formation, BioS-2P is a good choice as a scaffold for bone tissue engineering. (AU) | |
| Processo FAPESP: | 13/07793-6 - CeRTEV - Centro de Pesquisa, Tecnologia e Educação em Materiais Vítreos |
| Beneficiário: | Edgar Dutra Zanotto |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 12/23879-5 - Engenharia de tecidos: efeito da associação entre células e bio-sca sobre o reparo de defeitos ósseos |
| Beneficiário: | Adalberto Luiz Rosa |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/23525-9 - Engenharia de tecidos: efeito da associação entre células e bio-sca sobre o reparo de defeitos ósseo |
| Beneficiário: | Emanuela Prado Ferraz |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |