Transcriptomes from the photogenic and non-photogenetic tissues and life stages of the Aspisoma lineatum firefly (Coleoptera: Lampyridae): Implications for the evolutionary origins of bioluminescence and its associated light organs

Autor(es)

Amaral, Danilo T. ; Silva, Jaqueline R. ; Viviani, Vadim R. ^[1]

Afiliação do(s) autor(es)

^[1] Grad Sch Biotechnol & Environm Monitoring UFSCar, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENE REPORTS; v. 8, p. 150-159, SEP 2017.
• Citações Web of Science: 5

Resumo

In fireflies, the bioluminescent system involves a benzothiazolic luciferin, ATP, a luciferase, and complex lanterns consisting of different tissues including a photogenic one. Whereas the biochemical reaction and the structure of lanterns are quite well known, the evolutionary and developmental origins of photogenic tissue as well as the biosynthetic origin of luciferin remain enigmatic. One approach to better understand the origin of the photogenic tissue and biosynthetic origin of luciferin is to investigate the molecular differentiation among photogenic and non-photogenic tissues. Thus, here we compared - for the first time - the transcriptomes of the photogenic tissue of lanterns (specialized tissue) and the fat body (non-specialized tissue) of larvae, adult lanterns, and eggs stages of the common Southeastern Brazilian firefly Aspisoma lineatum. The similarities between the photogenic tissue of lanterns and the fat body, with the occurrence of several luciferase isozymes, luciferin-regenerating enzyme, L-DOPA metabolism related enzymes, and nitric oxide synthase, support the hypothesis that in fireflies, the photogenic tissue evolved from the fat body. (AU)

• Processo FAPESP: 14/20176-9 - Transcriptoma das lanternas de larva e adulto e do corpo gorduroso de larva de Elateroidea (Coleoptera)
• Beneficiário: Danilo Trabuco do Amaral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/09594-0 - Análise dos transcriptomas das laternas, corpo gorduroso e túbulos de Malpighi de coleópteros, e suas relações com as origens da bioluminescência em Coleoptera e Diptera
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular