Pathogenicity and genetic profile of oral Porphyromonas species from canine periodontitis

Autor(es)

Silva, Amanda do Nascimento ^[1] ; de Avila, Erica Dorigatti ^[2] ; Nakano, Viviane ^[1] ; Avila-Campos, Mario J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Anaerobe Lab, Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 83, p. 20-24, NOV 2017.
• Citações Web of Science: 2

Resumo

Objective: In this study, the presence of the prtC and fimA genes involved in the pathogenicity of oral Porphyromonas spp. isolated from dogs with periodontitis and healthy, as well as their genetic diversity was investigated. Design: Thirty-two Beagle dogs, 24 with periodontitis and 8 healthy were evaluated. Subgingival samples from only one gingival site of both groups were collected. Bacteria grown in anaerobiosis were identified by RAPID ID 32A kits. From each strain the respective DNA was obtained and used to genotyping by conventional PCR and AP-PCR. Results: Dogs with periodontitis harbored 28 P. gulae, 2 P. creviocaricanis, 1 P. cangingivalis and 7 P. macacae; and from healthy dogs, 11 P. gulae and 5 P. circumdentaria. In P. gulae isolated from periodontal dogs the gene prtC was observed in 19 (67.85%) and in 7 (63.63%) from healthy dogs. P. gulae strains from periodontal dogs harbored either the gene fimA I or fimA II; while strains from healthy dogs harbored the gene fimA I, fimA II, fimA III or fimA IV, as well as 1 P. circumdentaria the gene fimA II. By AP-PCR strains were grouped in different clusters suggesting heterogeneity of these microorganisms. Conclusions: The results presented herein inform that Porphyromonas spp. isolated from dogs with and without periodontitis harbored the prtC and fimA genes and it could be a role in the establishment of the infectious process. (AU)

• Processo FAPESP: 13/13652-6 - Determinação dos perfis fenotípicos e genotípicos em termos qualitativos e quantitativos de Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum isolados de diferentes condições clínicas bucais
• Beneficiário: Mario Júlio Ávila-Campos
• Linha de fomento: Auxílio à Pesquisa - Regular