Coupling of the ring-oven-based preconcentration technique and surface-enhanced Raman spectroscopy: Application for the determination of purine bases in DNA

Autor(es)

Villa, Javier E. L. ^[1] ; Pasquini, Celio ^[1] ; Poppi, Ronei J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13081970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 991, p. 95-103, OCT 23 2017.
• Citações Web of Science: 5

Resumo

In this paper, we present the advantages and limitations of the coupling of a ring-oven-based pre-concentration technique and surface-enhanced Raman spectroscopy (SERS). Three different methods to promote analyte adsorption on gold nanoparticles using crystal violet as a probe molecule were assessed. The results showed significant improvements in sampling process, selectivity, sensitivity, repeatability (less than +/- 10%), and detection limits (nanomolar level) using a sample volume as small as 300 mu L. Finally, the standard addition method was successfully applied to the quantitative SERS detection of adenine and guanine in calf thymus DNA after ring-oven preconcentration with a calculated value of (G + C)/(A + T) close to the literature value. This work could therefore pave the way to quantifying a wide variety of biologically relevant compounds in real-world samples via the use of a biodegradable, low-cost and disposable paper platform for SERS. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/57808-1 - Instituto Nacional de Ciências e Tecnologias Analíticas Avançadas - INCTAA
• Beneficiário: Celio Pasquini
• Modalidade de apoio: Auxílio à Pesquisa - Temático