Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity

Autor(es)

Alcantara dos Santos, Sergio Alexandre ^[1] ; Porto Amorim, Elaine Manoela ^[2] ; Ribeiro, Larissa Mayume ^[1] ; Rinaldi, Jaqueline Carvalho ^[3] ; Delella, Flavia Karma ^[2] ; Justulin, Luis Antonio ^[1] ; Felisbino, Sergio Luis ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dept Morphol, Inst Biosci, BR-18618689 Botucatu, SP - Brazil

^[2] State Univ West Parana UNIGESTE, Ctr Biol & Hlth Sci CCBS, Cascavel, Parana - Brazil

^[3] State Univ Maringa UEM, Biol Sci Ctr, Dept Morphol Sci, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 493, n. 4, p. 1581-1586, DEC 2 2017.
• Citações Web of Science: 0

Resumo

Puberty is an important period for the growth and maturation of the male reproductive system, and is also a critical window for endocrine or environmental interference. The physiological levels of circulating insulin and hyperglycemic control are important factors for a normal prostate growth. Hyperglycemia during puberty is reported to retard the growth of the prostate gland, with remarkable effects on the epithelial compartment. Here, we investigated the impact of hyperglycemia along with a simultaneous or late insulin replacement on the ventral prostate growth in rats during puberty, paying special attention to the deposition of collagen fibers and activities of gelatinase, matrix metalloproteinase-2 (MMP-2), and -9 (MMP-9). Hyperglycemia was induced by streptozotocin (STZ) administration in 40-day-old male Wistar rats. A subset of hyperglycemic rats underwent an early insulin replacement (three days after the STZ administration), and another subset underwent a late insulin replacement (twenty days after the STZ administration). Animals were euthanized at 60 and/or 80 days of age. The ventral prostatic lobe was processed for picrosirius red staining, type I and III collagen immunohistochemistry, and gelatin zymography. Hyperglycemic animals showed an increased area of collagen fibers in the prostate, which was composed both types of collagens. MMP-2 activity was significantly reduced in the hyperglycemic animals, while MMP-9 activity was very low and showed no alteration. The simultaneous and late insulin administration restored collagen content and MMP-2 activity. In conclusion, puberty is a critical window for prostate maturation and type-I diabetes-induced hyperglycemia affects the ratio of the prostatic parenchymal and stromal growth, leading to fibrotic tissues by also MMP-2 down regulation. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/60115-2 - Efeito do diabete e da reposicao com insulina sobre o processo de maturacao da prostata ventral do rato wistar: alteracoes morfologicas e na atividade de metaloproteinases de matriz mmp-2 e -9.
• Beneficiário: Sérgio Luis Felisbino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/60116-9 - Efeito do diabete e da reposicao com insulina sobre o processo de maturacao da prostata ventral do rato wistar: alteracoes morfologicas e na atividade de metaloproteinases de matriz mmp-2 e -9.
• Beneficiário: Elaine Manoela Porto
• Modalidade de apoio: Bolsas no Brasil - Doutorado