Mesenchymal-like stem cells in canine ovary show h... - BV FAPESP
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Mesenchymal-like stem cells in canine ovary show high differentiation potential

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Autor(es):
Trindade, A. B. [1, 2] ; Therrien, J. [2] ; Garcia, J. M. [1] ; Smith, L. C. [2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil
[2] Univ Montreal, Ctr Rech Reprod & Fertilite, Fac Med Vet, St Hyacinthe, PQ - Canada
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Cell Proliferation; v. 50, n. 6 DEC 2017.
Citações Web of Science: 3
Resumo

Objectives: Recent studies have reported the existence of stem cells in ovarian tissue that show enhanced proliferative and differentiation potential compared to other adult tissues. Based on this evidence, we hypothesized that ovarian tissue contained mesenchymal-like stem cells (MSC) that could be isolated using a novel rapid plastic adhesion technique. Materials and methods: We established MSC lines derived from ovarian and adipose tissue based on their ability to rapidly adhere to plastic culture dishes in the first 3 hours after plating and studied their potentiality in terms of molecular markers and differentiation capacity. Results: Morphological and kinetic properties of in vitro cultured ovarian MSC were similar to adipose-derived MSC, and both reached senescence after similar passage numbers. Ovarian-derived MSC expressed mesenchymal (CD90 and CD44) but not haematopoietic markers (CD34 and CD45), indicating similarity to adipose-derived MSC. Moreover, ovarian-derived MSC expressed NANOG, TERT, SOX2, OCT4 and showed extensive capacity to differentiate not only into adipogenic, osteogenic and chondrogenic tissue but also towards neurogenic and endodermal lineages and even precursors of primordial germ cells. Conclusion: These results show for the first time the derivation of ovarian cells with the molecular properties of MSC as well as wide differentiation potential. Canine ovarian tissue is accessible, expandable, multipotent and has high plasticity, holding promise for applications in regenerative medicine. (AU)

Processo FAPESP: 13/14293-0 - Isolamento e caracterização de células tronco derivadas do tecido ovariano de cães adultos
Beneficiário:Amanda Baracho Trindade Hill
Modalidade de apoio: Bolsas no Brasil - Mestrado