Raman spectroscopy applied to identify metabolites in urine of physically active subjects

Autor(es)

Moreira, Leticia Parada ^[1] ; Silveira, Jr., Landulfo ^{[1, 2]} ; da Silva, Alexandre Galvao ^[1] ; Fernandes, Adriana Barrinha ^[2] ; Tavares Pacheco, Marcos Tadeu ^{[1, 2]} ; Ferraretto Moura Rocco, Debora Dias ^[1]

Afiliação do(s) autor(es)

^[1] Univ Santa Cecilia UNISANTA, Rua Oswaldo Cruz 277, BR-11045907 Santos, SP - Brazil

^[2] UAM, CITE, Parque Tecnol Sao Jose dos Campos, BR-12247016 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 176, p. 92-99, NOV 2017.
• Citações Web of Science: 6

Resumo

Raman spectroscopy is a rapid and non-destructive technique suitable for biological fluids analysis. In this work, dispersive Raman spectroscopy has been employed as a rapid and nondestructive technique to detect the metabolites in urine of physically active subjects before and after vigorous 30 min pedaling or running compared to sedentary subjects. For so, urine samples from 9 subjects were obtained before and immediately after physical activities and submitted to Raman spectroscopy (830 nm excitation, 250 mW laser power, 20 s integration time) and compared to urine from 5 sedentary subjects. The Raman spectra of urine from sedentary showed peaks related to urea, creatinine, ketone bodies, phosphate and other nitrogenous compounds. These metabolic biomarkers presented peaks with different intensities in the urine of physically active individuals after exercises compared to before, measured by the intensity of selected peaks the Raman spectra, which means different concentrations after training. These peaks presented different intensity values for each subject before physical activity, also behaving differently compared to the post-training: some subjects presented increase while others decrease the intensity. Raman spectroscopy may allow the development of a rapid and non-destructive test for metabolic evaluation of the physical training in active and trained subjects using urine samples, allowing nutrition adjustment with the spores performance. (AU)

• Processo FAPESP: 09/01788-5 - Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata
• Beneficiário: Landulfo Silveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular