Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata

• Processo: 09/01788-5
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Vigência: 01 de setembro de 2009 - 29 de fevereiro de 2012
• Área do conhecimento: Engenharias - Engenharia Biomédica - Bioengenharia
• Pesquisador responsável: Landulfo Silveira Junior
• Beneficiário: Landulfo Silveira Junior
• Instituição Sede: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade Camilo Castelo Branco (UNICASTELO). São Paulo , SP, Brasil
• Assunto(s): Neoplasias Espectroscopia Raman
• Palavra(s)-Chave do Pesquisador: Componentes bioquímicos | espectroscopia Raman | Fibras Opticas | Laser Diagnóstico | quantificação | tecido biológico | Diagnóstico

Resumo

Este projeto de pesquisa objetiva a aplicação da técnica de espectroscopia Raman dispersiva na identificação das alterações bioquímicas relacionadas com as patologias neoplásicas em tecidos biológicos de pele humana in vitro e in vivo e próstata in vitro. Pretende-se com a técnica obter uma informação de diagnóstico que possibilite identificar e classificar os tecidos biológicos alterados dos normais, utilizando algoritmos de diagnóstico baseados em estatística multivariada como a PCA e em um modelo que considere os componentes bioquímicos básicos e morfológicos dos tecidos responsáveis pelo espectro observado, uma vez que os tecidos biológicos exibem assinatura espectral dos componentes bioquímicos presentes, com alta sensibilidade e especificidade. Com a utilização de um sistema Raman dispersivo com excitação na região do infravermelho próximo (830 nm) acoplado a um cabo de fibras ópticas ‘Raman probe’ ou a um microscópio (Micro-Raman), obtém-se um espectro com reduzida fluorescência da amostra e com minimização do background gerado pelo Raman da fibra óptica, abrindo a possibilidade de realização de estudos in vivo em tecidos biológicos e correlacioná-los com os resultados in vitro, fornecendo subsídios para o desenvolvimento de um equipamento de auxílio ao diagnóstico de lesões neoplásicas. O sistema é composto por um espectrógrafo com excitação por laser semicondutor (830nm), um ‘Raman probe’ que excita e coleta o sinal Raman do tecido, e o microscópio, utilizado para obter espectros das estruturas morfológicas da pele e correlacionar com as alterações bioquímicas. O projeto pretende estudar amostras de câncer de pele e de próstata in vitro e de lesões cutâneas in vivo, analisadas via algoritmo de diagnóstico baseado em PCA e análise discriminante e em cálculo das concentrações relativas dos componentes bioquímicos mais importantes dos tecidos, viabilizando a técnica Raman para seu uso na área de diagnóstico de doenças. (AU)

Publicações científicas

(62)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

GIANSANTE, STELLA; GIANA, HECTOR ENRIQUE; FERNANDES, ADRIANA BARRINHA; SILVEIRA, JR., LANDULFO. Analytical performance of Raman spectroscopy in assaying biochemical components in human serum. Lasers in Medical Science, FEB 2021. (09/01788-5)

SILVEIRA, JR., LANDULFO; SILVEIRA, FABRICIO LUIZ; BODANESE, BENITO; ZANGARO, RENATO AMARO; PACHECO, MARCOS TADEU T.. Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals. Journal of Biomedical Optics, v. 17, n. 7, JUL 2012. (09/01788-5)

BODANESE, BENITO; SILVEIRA, FABRICIO LUIZ; ZANGARO, RENATO AMARO; PACHECO, MARCOS TADEU T.; PASQUALUCCI, CARLOS AUGUSTO; SILVEIRA, JR., LANDULFO. Discrimination of Basal Cell Carcinoma and Melanoma from Normal Skin Biopsies in Vitro Through Raman Spectroscopy and Principal Component Analysis. Photomedicine and Laser Surgery, v. 30, n. 7, p. 381-387, JUL 2012. (09/01788-5)

PASSONI, DOUGLAS DE JESUS; PACHECO, MARCOS TADEU T.; SILVEIRA JR, LANDULFO. Raman spectroscopy for the identification of differences in the composition of automobile lubricant oils related to SAE specifications and additives. Instrumentation Science & Technology, v. 49, n. 2, AUG 2020. (09/01788-5)

DE M. BEZERRA, ANDRESSA CRISTINA; DE A. COELHO, NELIZE MARIA; BERTELLI, FELIPE; PACHECO, MARCOS TADEU T.; SILVEIRA, JR., LANDULFO. Temperature-Induced Chemical Changes in Lubricant Automotive Oils Evaluated Using Raman Spectroscopy. Applied Spectroscopy, v. 75, n. 2, SEP 2020. (09/01788-5)

DA SILVA, ADRIANO MORAES; ANA DE SIQUEIRA E OLIVEIRA, FERNANDA SANT; DE BRITO, PEDRO LUIZ; SILVEIRA JR, LANDULFO. Spectral model for diagnosis of acute leukemias in whole blood and plasma through Raman spectroscopy. Journal of Biomedical Optics, v. 23, n. 10, OCT 2018. (09/01788-5)

GIANSANTE, STELLA; GIANA, HECTOR ENRIQUE; FERNANDES, ADRIANA BARRINHA; SILVEIRA, JR., LANDULFO. nalytical performance of Raman spectroscopy in assaying biochemical components in human seru. Lasers in Medical Science, v. 37, n. 1, p. 287-298, FEB 2022. (09/01788-5)

AGUIAR, RICARDO PINTO; FALCAO, EDGAR TEIXEIRA; PASQUALUCCI, CARLOS AUGUSTO; SILVEIRA, JR., LANDULFO. se of Raman spectroscopy to evaluate the biochemical composition of normal and tumoral human brain tissues for diagnosi. Lasers in Medical Science, v. 37, n. 1, p. 121-133, FEB 2022. (09/01788-5)

SILVEIRA JR, LANDULFO; PASQUALUCCI, CARLOS AUGUSTO; BODANESE, BENITO; TAVARES PACHECO, MARCOS TADEU; ZANGARO, RENATO AMARO. Normal-subtracted preprocessing of Raman spectra aiming to discriminate skin actinic keratosis and neoplasias from benign lesions and normal skin tissues. Lasers in Medical Science, v. 35, n. 5, p. 1141-1151, JUL 2020. (09/01788-5)

DE ALMEIDA, MAURICIO LIBERAL; SAATKAMP, CASSIANO JUNIOR; FERNANDES, ADRIANA BARRINHA; BARBOSA PINHEIRO, ANTONIO LUIZ; SILVEIRA, JR., LANDULFO. Estimating the concentration of urea and creatinine in the human serum of normal and dialysis patients through Raman spectroscopy. Lasers in Medical Science, v. 31, n. 7, p. 1415-1423, SEP 2016. (09/01788-5)

BORIO, VIVIANE GADRET; FERNANDES, ADJACI UCHOA; SILVEIRA, JR., LANDULFO. Characterization of an ultraviolet irradiation chamber to monitor molecular photo degradation by Raman spectroscopy. Instrumentation Science & Technology, v. 44, n. 2, p. 189-198, MAR 3 2016. (09/01788-5)

RITA DE CÁSSIA FERNANDES BORGES; RICARDO SCARPARO NAVARRO; HECTOR ENRIQUE GIANA; FERNANDA GRUBISICH TAVARES; ADRIANA BARRINHA FERNANDES; LANDULFO SILVEIRA JUNIOR. Detecting alterations of glucose and lipid components in human serum by near-infrared Raman spectroscopy. Res. Biomed. Eng., v. 31, n. 2, p. 160-168, 2015-04-00. (09/01788-5)

MOURA JUNIOR, MANOEL DE JESUS; MARTINS MAIA FILHO, ANTONIO LUIS; PESSOA, DIEGO RODRIGUES; RODRIGUES ALVES, MARCOS DANILO; JUSTINO, JEFFERSON DE SOUSA; ANDRADE, MATHEUS DOS SANTOS; GONCALVES REBELO, ANA MARIA; DE LIMA, CARLOS JOSE; BARBOSA PINHEIRO, ANTONIO LUIZ; SILVEIRA, JR., LANDULFO. Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy. Lasers in Medical Science, v. 30, n. 6, p. 1729-1738, AUG 2015. (09/01788-5)

PENIDO, CIRO A. F. O.; PACHECO, MARCOS TADEU T.; ZANGARO, RENATO A.; SILVEIRA, JR., LANDULFO. Identification of Different Forms of Cocaine and Substances Used in Adulteration Using Near-infrared Raman Spectroscopy and Infrared Absorption Spectroscopy. JOURNAL OF FORENSIC SCIENCES, v. 60, n. 1, p. 171-178, JAN 2015. (09/01788-5)

MANGUEIRA, NILTON MACIEL; XAVIER, MURILO; DE SOUZA, RENATO APARECIDO; CASTILLO SALGADO, MIGUEL ANGEL; SILVEIRA, JR., LANDULFO; VILLAVERDE, ANTONIO BALBIN. Effect of Low-Level Laser Therapy in an Experimental Model of Osteoarthritis in Rats Evaluated Through Raman Spectroscopy. Photomedicine and Laser Surgery, v. 33, n. 3, p. 145-153, MAR 1 2015. (09/01788-5)

DE SIQUEIRA E OLIVEIRA, FERNANDA SANTANA; GIANA, HECTOR ENRIQUE; SILVEIRA, JR., LANDULFO. Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and principal components analysis. Journal of Biomedical Optics, v. 17, n. 10, OCT 2012. (09/01788-5)

AGUIAR, RICARDO PINTO; SILVEIRA, JR., LANDULFO; FALCAO, EDGAR TEIXEIRA; TAVARES PACHECO, MARCOS TADEU; ZANGARO, RENATO AMARO; PASQUALUCCI, CARLOS AUGUSTO. Discriminating Neoplastic and Normal Brain Tissues in Vitro Through Raman Spectroscopy: A Principal Components Analysis Classification Model. Photomedicine and Laser Surgery, v. 31, n. 12, p. 595-604, DEC 1 2013. (09/01788-5)

MOREIRA, LETICIA PARADA; FERRARETTO MOURA ROCCO, DEBORA DIAS; DA SILVA, ALEXANDRE GALVAO; TAVARES PACHECO, MARCOS TADEU; SILVEIRA JR, LANDULFO. Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy. Lasers in Medical Science, v. 35, n. 2, p. 455-464, MAR 2020. (09/01788-5)

SAATKAMP, CASSIANO JUNIOR; DE ALMEIDA, MAURICIO LIBERAL; MARTINS BISPO, JEYSE ALIANA; BARBOSA PINHEIRO, ANTONIO LUIZ; FERNANDES, ADRIANA BARRINHA; SILVEIRA, JR., LANDULFO. Quantifying creatinine and urea in human urine through Raman spectroscopy aiming at diagnosis of kidney disease. Journal of Biomedical Optics, v. 21, n. 3, MAR 2016. (09/01788-5)

FERNANDES DE OLIVEIRA PENIDO, CIRO AUGUSTO; TAVARES PACHECO, MARCOS TADEU; NOVOTNY, ETELVINO HENRIQUE; LEDNEV, IGOR K.; SILVEIRA, JR., LANDULFO. Quantification of cocaine in ternary mixtures using partial least squares regression applied to Raman and Fourier transform infrared spectroscopy. Journal of Raman Spectroscopy, v. 48, n. 12, p. 1732-1743, DEC 2017. (09/01788-5, 15/24040-7)

DE SOUZA, RENATO APARECIDO; XAVIER, MURILO; MANGUEIRA, NILTON MACIEL; SANTOS, ANA PAULA; BARBOSA PINHEIRO, ANTONIO LUIZ; VILLAVERDE, ANTONIO BALBIN; SILVEIRA, LANDULFO, JR.. Raman spectroscopy detection of molecular changes associated with two experimental models of osteoarthritis in rats. Lasers in Medical Science, v. 29, n. 2, p. 8-pg., 2014-03-01. (09/01788-5)

SOUSA VIEIRA, E. E.; SILVEIRA JUNIOR, L.; BARRINHA FERNANDES, A.; BASTOS-FILHO, TF; CALDEIRA, EMD; FRIZERA-NETO, A. Differential Diagnosis of Glycosuria Using Raman Spectroscopy. XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020, v. N/A, p. 6-pg., 2022-01-01. (09/01788-5)

MARRAFA, P. A. L., I; CARVALHO, H. C.; LIMA, C. J.; MORETTI, A. B. F.; SILVEIRA JR, L.; BASTOS-FILHO, TF; CALDEIRA, EMD; FRIZERA-NETO, A. Dispersive Raman Spectroscopy of Peanut Oil-Ozone and Ultrasound Effects. XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020, v. N/A, p. 5-pg., 2022-01-01. (09/01788-5)

SILVA, MARCOS A. S. R.; LOPES, RICARDO M.; AIMBIRE, FLAVIO; LEITE, KATIA R. M.; PASQUALUCCI, CARLOS A.; PACHECO, MARCOS T. T.; SILVEIRA, LANDULFO, JR.; CHAMPION, PM; ZIEGLER, LD. Diagnostic Model for Differentiating Human Malignant Prostate Lesion from Normal Tissue in Vitro by Raman Spectroscopy. MAGNETIC FIELDS IN THE UNIVERSE: FROM LABORATORY AND STARS TO PRIMORDIAL STRUCTURES, v. 1267, p. 2-pg., 2010-01-01. (09/01788-5)

BODANESE, BENITO; SILVEIRA, LANDULFO, JR.; ALBERTINI, REGIANE; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU. Differentiating Normal and Basal Cell Carcinoma Human Skin Tissues In Vitro Using Dispersive Raman Spectroscopy: A Comparison Between Principal Components Analysis and Simplified Biochemical Models. Photomedicine and Laser Surgery, v. 28, p. 9-pg., 2010-08-01. (09/01788-5)

DE SIQUEIRA E OLIVEIRA, FERNANDA S.; GIANA, HECTOR E.; SILVEIRA, LANDULFO, JR.; VODINH, T; MAHADEVANJANSEN, A; GRUNDFEST, WS. Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and Principal Components Analysis. ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSTIC SYSTEMS X, v. 8214, p. 7-pg., 2012-01-01. (09/01788-5)

SILVEIRA, LANDULFO, JR.; SILVEIRA, FABRICIO L.; BODANESE, BENITO; PACHECO, MARCOS TADEU T.; ZANGARO, RENATO A.; KOLLIAS, N; CHOI, B; ZENG, H; KANG, HW; KNUDSEN, BE; et al. Diagnosing basal cell carcinoma in vivo by near-infrared Raman spectroscopy: a Principal Components Analysis discrimination algorithm. PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2, v. 8207, p. 7-pg., 2012-01-01. (10/11111-0, 09/01788-5)

LIMA, ANA MARA FERREIRA; DANIEL, CAMILA RIBEIRO; PACHECO, MARCOS TADEU TAVARES; DE BRITO, PEDRO LUIZ; SILVEIRA, LANDULFO. Discrimination of leukemias and non-leukemic cancers in blood serum samples of children and adolescents using a Raman spectral model. Lasers in Medical Science, v. 38, n. 1, p. 13-pg., 2022-12-24. (09/01788-5)

AGUIAR, RICARDO PINTO; FALCAO, EDGAR TEIXEIRA; PASQUALUCCI, CARLOS AUGUSTO; SILVEIRA, JR., LANDULFO. Use of Raman spectroscopy to evaluate the biochemical composition of normal and tumoral human brain tissues for diagnosis. Lasers in Medical Science, NOV 2020. (09/01788-5)

DA SILVA, WAGNER RAFAEL; SILVEIRA JR, LANDULFO; FERNANDES, ADRIANA BARRINHA. Diagnosing sickle cell disease and iron deficiency anemia in human blood by Raman spectroscopy. Lasers in Medical Science, OCT 2019. (09/01788-5)

DE SOUZA, RENATO APARECIDO; XAVIER, MURILO; MANGUEIRA, NILTON MACIEL; SANTOS, ANA PAULA; BARBOSA PINHEIRO, ANTONIO LUIZ; VILLAVERDE, ANTONIO BALBIN; SILVEIRA, JR., LANDULFO. Raman spectroscopy detection of molecular changes associated with two experimental models of osteoarthritis in rats. Lasers in Medical Science, v. 29, n. 2, SI, p. 797-804, MAR 2014. (09/01788-5)

GUIMARAES, LUCIANA LOPES; MOREIRA, LETICIA PARADA; LOURENCO, BARBARA FARIA; TOMA, WALBER; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU; SILVEIRA, JR., LANDULFO. Multivariate Method Based on Raman Spectroscopy for Quantification of Dipyrone in Oral Solutions. JOURNAL OF SPECTROSCOPY, 2018. (09/01788-5)

FERNANDES DE OLIVEIRA PENIDO, CIRO AUGUSTO; TAVARES PACHECO, MARCOS TADEU; LEDNEV, IGOR K.; SILVEIRA, JR., LANDULFO. Raman spectroscopy in forensic analysis: identification of cocaine and other illegal drugs of abuse. Journal of Raman Spectroscopy, v. 47, n. 1, SI, p. 28-38, JAN 2016. (09/01788-5)

BODANESE, BENITO; SILVEIRA, JR., LANDULFO; ALBERTINI, REGIANE; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU. Differentiating Normal and Basal Cell Carcinoma Human Skin Tissues In Vitro Using Dispersive Raman Spectroscopy: A Comparison Between Principal Components Analysis and Simplified Biochemical Models. Photomedicine and Laser Surgery, v. 28, n. 1, p. S119-S127, AUG 2010. (09/01788-5)

CASTRO GOULART, ANA CRISTINA; ZANGARO, RENATO AMARO; CARVALHO, HENRIQUE CUNHA; SILVEIRA JR, LANDULFO. iagnosing COVID-19 in human sera with detected immunoglobulins IgM and IgG by means of Raman spectroscop. Journal of Raman Spectroscopy, v. 52, n. 12, SI, AUG 2021. (09/01788-5)

MELO MACIEL MANGUEIRA, MELISSA DE ALMEIDA; MANGUEIRA, NILTON MACIEL; GAMA FILHO, OZIMO PEREIRA; DE OLIVEIRA, MARCIO MOYSES; HELUY, RENATO ALBUQUERQUE; SILVEIRA, JR., LANDULFO; MONIZ DE ARAGAO DAQUER, EGAS CAPARELLI. Biochemical changes in injured sciatic nerve of rats after low-level laser therapy (660nm and 808nm) evaluated by Raman spectroscopy. Lasers in Medical Science, v. 34, n. 3, p. 525-535, APR 2019. (09/01788-5)

MOREIRA, LETICIA PARADA; SILVEIRA, JR., LANDULFO; TAVARES PACHECO, MARCOS TADEU; DA SILVA, ALEXANDRE GALVAO; FERRARETTO MOURA ROCCO, DEBORA DIAS. Detecting urine metabolites related to training performance in swimming athletes by means of Raman spectroscopy and principal component analysis. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 185, p. 223-234, AUG 2018. (09/01788-5)

BARBOSA PINHEIRO, ANTONIO LUIZ; PINHEIRO SOARES, LUIZ GUILHERME; CORDEIRO MARQUES, APARECIDA MARIA; TEIXEIRA CANGUSSU, MARIA CRISTINA; TAVARES PACHECO, MARCOS TADEU; SILVEIRA, JR., LANDULFO. Biochemical changes on the repair of surgical bone defects grafted with biphasic synthetic micro-granular HA plus beta-tricalcium phosphate induced by laser and LED phototherapies and assessed by Raman spectroscopy. Lasers in Medical Science, v. 32, n. 3, p. 663-672, APR 2017. (15/24040-7, 09/01788-5)

SILVEIRA, JR., LANDULFO; MARTINS MOTTA, EDLENE DO CARMO; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU; DE LIMA, CARLOS JOSE; MOREIRA, LIVIA HELENA. Characterization of nutritional parameters in bovine milk by Raman spectroscopy with least squares modeling. Instrumentation Science & Technology, v. 44, n. 1, p. 85-97, JAN 2 2016. (09/01788-5)

TOMA, WALBER; GUIMARAES, LUCIANA L.; BRITO, ALBA R. M. S.; SANTOS, ALDO R.; CORTEZ, FERNANDO S.; PUSCEDDU, FABIO H.; CESAR, AUGUSTO; JUNIOR, LANDULFO S.; PACHECO, MARCOS T. T.; PEREIRA, CAMILO D. S.. Safflower oil: an integrated assessment of phytochemistry, antiulcerogenic activity, and rodent and environmental toxicity. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 24, n. 5, p. 538-544, SEP-OCT 2014. (09/01788-5)

SILVEIRA, JR., LANDULFO; LEITE, KATIA RAMOS M.; SILVEIRA, FABRICIO LUIZ; SROUGI, MIGUEL; PACHECO, MARCOS TADEU T.; ZANGARO, RENATO AMARO; PASQUALUCCI, CARLOS AUGUSTO. Discrimination of prostate carcinoma from benign prostate tissue fragments in vitro by estimating the gross biochemical alterations through Raman spectroscopy. Lasers in Medical Science, v. 29, n. 4, p. 1469-1477, JUL 2014. (09/01788-5, 12/20666-0)

PINHEIRO, ANTONIO LUIZ B.; SOARES, LUIZ GUILHERME P.; MARQUES, APARECIDA MARIA C.; ACIOLE, JOUBER MATEUS S.; DE SOUZA, RENATO APARECIDO; SILVEIRA, JR., LANDULFO. Raman ratios on the repair of grafted surgical bone defects irradiated or not with laser (lambda 780 nm) or LED (lambda 850 nm). JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 138, p. 146-154, SEP 5 2014. (09/01788-5)

DE SOUZA VIEIRA, ELZO EVERTON; MARTINS BISPO, JEYSE ALIANA; SILVEIRA, JR., LANDULFO; FERNANDES, ADRIANA BARRINHA. Discrimination model applied to urinalysis of patients with diabetes and hypertension aiming at diagnosis of chronic kidney disease by Raman spectroscopy. Lasers in Medical Science, v. 32, n. 7, p. 1605-1613, SEP 2017. (09/01788-5)

MUSSATTO, JULIO CESAR; PEREZ, MAURO COURA; DE SOUZA, RENATO APARECIDO; PACHECO, MARCOS TADEU T.; ZANGARO, RENATO AMARO; SILVEIRA, JR., LANDULFO. Could the bone mineral density (T-score) be correlated with the Raman spectral features of keratin from women's nails and be used to predict osteoporosis?. Lasers in Medical Science, v. 30, n. 1, p. 287-294, JAN 2015. (09/01788-5)

SILVEIRA, FABRICIO L.; PACHECO, MARCOS T. T.; BODANESE, BENITO; PASQUALUCCI, CARLOS A.; ZANGARO, RENATO A.; SILVEIRA, JR., LANDULFO. Discrimination of Non-Melanoma Skin Lesions From Non-Tumor Human Skin Tissues In Vivo Using Raman Spectroscopy and Multivariate Statistics. Lasers in Surgery and Medicine, v. 47, n. 1, p. 6-16, JAN 2015. (10/11111-0, 12/20666-0, 09/01788-5)

FERNANDES DE OLIVEIRA PENIDO, CIRO AUGUSTO; SILVEIRA, JR., LANDULFO; TAVARES PACHECO, MARCOS TADEU. QUANTIFICATION OF BINARY MIXTURES OF COCAINE AND ADULTERANTS USING DISPERSIVE RAMAN AND FT-IR SPECTROSCOPY AND PRINCIPAL COMPONENT REGRESSION. Instrumentation Science & Technology, v. 40, n. 5, p. 441-456, 2012. (09/01788-5)

MOREIRA, LETICIA PARADA; SILVEIRA, JR., LANDULFO; DA SILVA, ALEXANDRE GALVAO; FERNANDES, ADRIANA BARRINHA; TAVARES PACHECO, MARCOS TADEU; FERRARETTO MOURA ROCCO, DEBORA DIAS. Raman spectroscopy applied to identify metabolites in urine of physically active subjects. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 176, p. 92-99, NOV 2017. (09/01788-5)

FERREIRA LIMA, ANA MARA; DANIEL, CAMILA RIBEIRO; NAVARRO, RICARDO SCARPARO; BODANESE, BENITO; PASQUALUCCI, CARLOS AUGUSTO; TAVARES PACHECO, MARCOS TADEU; ZANGARO, RENATO AMARO; SILVEIRA JR, LANDULFO. Discrimination of non-melanoma skin cancer and keratosis from normal skin tissue in vivo and ex vivo by Raman spectroscopy. VIBRATIONAL SPECTROSCOPY, v. 100, p. 131-141, JAN 2019. (09/01788-5)

SILVEIRA, JR., LANDULFO; FERNANDES BORGES, RITA DE CASSIA; NAVARRO, RICARDO SCARPARO; GIANA, HECTOR ENRIQUE; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU; FERNANDES, ADRIANA BARRINHA. Quantifying glucose and lipid components in human serum by Raman spectroscopy and multivariate statistics. Lasers in Medical Science, v. 32, n. 4, p. 787-795, MAY 2017. (09/01788-5)

LOPES, RICARDO MATIAS; SILVEIRA, JR., LANDULFO; SILVA, MARCOS AUGUSTO R. S.; LEITE, KATIA RAMOS M.; PASQUALUCCI, CARLOS AUGUSTO G.; PACHECO, MARCOS TADEU T.. Diagnostic model based on Raman spectra of normal, hyperplasia and prostate adenocarcinoma tissues in vitro. SPECTROSCOPY-AN INTERNATIONAL JOURNAL, v. 25, n. 2, p. 89-102, 2011. (09/01788-5)

SILVEIRA, JR., LANDULFO; BODANESE, BENITO; ZANGARO, RENATO AMARO; PACHECO, MARCOS TADEU T.. DISCRETE WAVELET TRANSFORM FOR DENOISING RAMAN SPECTRA OF HUMAN SKIN TISSUES USED IN A DISCRIMINANT DIAGNOSTIC ALGORITHM. Instrumentation Science & Technology, v. 38, n. 4, p. 268-282, 2010. (09/01788-5)

DE SIQUEIRA E OLIVEIRA, FERNANDA SANTANA; DA SILVA, ADRIANO MORAES; TAVARES PACHECO, MARCOS TADEU; GIANA, HECTOR ENRIQUE; SILVEIRA JR, LANDULFO. Biochemical characterization of pathogenic bacterial species using Raman spectroscopy and discrimination model based on selected spectral features. Lasers in Medical Science, v. 36, n. 2, JUN 2020. (09/01788-5)

GOULART, ANA CRISTINA CASTRO; ZANGARO, RENATO AMARO; CARVALHO, HENRIQUE CUNHA; LEDNEV, IGOR K.; SILVEIRA, LANDULFO. Diagnosing COVID-19 in nasopharyngeal secretion through Raman spectroscopy: a feasibility study. Lasers in Medical Science, v. 38, n. 1, p. 8-pg., 2023-09-12. (09/01788-5)

FERNANDES DE OLIVEIRA PENIDO, CIRO AUGUSTO; TAVARES PACHECO, MARCOS TADEU; LEDNEV, IGOR K.; SILVEIRA, LANDULFO, JR.. Raman spectroscopy in forensic analysis: identification of cocaine and other illegal drugs of abuse. Journal of Raman Spectroscopy, v. 47, n. 1, p. 11-pg., 2016-01-01. (09/01788-5)

MELITTO, ALEXANDRE S.; ARIAS, VICTOR E. A.; SHIDA, JORGE Y.; GEBRIM, LUIZ H.; SILVEIRA, LANDULFO, JR.. Diagnosing molecular subtypes of breast cancer by means of Raman spectroscopy. Lasers in Surgery and Medicine, v. 54, n. 8, p. 14-pg., 2022-07-05. (09/01788-5)

CARVALHO, GIZELA; CARVALHO, HENRIQUE C.; ROCCO, DEBORA D. F. M.; MOREIRA, LETICIA P.; PACHECO, MARCOS TADEU T.; SILVEIRA, LANDULFO, JR.; COSTAFELIX, R; MACHADO, JC; ALVARENGA, AV. Identification of Metabolites in Urine of Physical Exercise Practitioners by Raman Spectroscopy. XXVI BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2018, VOL. 2, v. 70, n. 2, p. 4-pg., 2019-01-01. (09/01788-5)

SILVEIRA, LANDULFO, JR.; LEITE, KATIA RAMOS M.; SROUGI, MIGUEL; SILVEIRA, FABRICIO L.; PACHECO, MARCOS TADEU T.; PASQUALUCCI, CARLOS A.; KOLLIAS, N; CHOI, B; ZENG, H; KANG, HW; et al. Could Raman spectroscopy discriminate the biochemical alterations among prostate carcinoma and benign prostate tissues? An in vitro study. PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2, v. 8207, p. 7-pg., 2012-01-01. (09/01788-5)

DA SILVA, WAGNER RAFAEL; SILVEIRA JR, LANDULFO; FERNANDES, ADRIANA BARRINHA. Diagnosing sickle cell disease and iron deficiency anemia in human blood by Raman spectroscopy. Lasers in Medical Science, v. 35, n. 5, p. 10-pg., 2019-10-22. (09/01788-5)

GUIMARAES, LUCIANA LOPES; MOREIRA, LETICIA PARADA; LOURENCO, BARBARA FARIA; TOMA, WALBER; ZANGARO, RENATO AMARO; TAVARES PACHECO, MARCOS TADEU; SILVEIRA, LANDULFO, JR.. Multivariate Method Based on Raman Spectroscopy for Quantification of Dipyrone in Oral Solutions. JOURNAL OF SPECTROSCOPY, v. 2018, p. 10-pg., 2018-01-01. (09/01788-5)

DE ALMEIDA MELO MACIEL MANGUEIRA, MELISSA; CAPARELLI-DAQUER, EGAS; FILHO, OZIMO PEREIRA GAMA; DE ASSIS, DIOGO SOUZA FERREIRA RUBIM; SOUSA, JANYEID KARLA CASTRO; LIMA, WILLY LEITE; PINHEIRO, ANTONIO LUIZ BARBOSA; SILVEIRA, LANDULFO, JR.; MANGUEIRA, NILTON MACIEL. Raman spectroscopy and sciatic functional index (SFI) after low-level laser therapy (LLLT) in a rat sciatic nerve crush injury model. Lasers in Medical Science, v. 37, n. 7, p. 15-pg., 2022-05-03. (09/01788-5)

DE SOUSA VIEIRA, ELZO EVERTON; SILVEIRA JR, LANDULFO; CARVALHO, HENRIQUE CUNHA; MARTINS BISPO, JEYSE ALIANA; FERNANDES, FERNANDA BARRINHA; FERNANDES, ADRIANA BARRINHA. Biochemical Analysis of Urine Samples from Diabetic and Hypertensive Patients without Renal Dysfunction Using Spectrophotometry and Raman Spectroscopy Techniques Aiming Classification and Diagnosis. BIOENGINEERING-BASEL, v. 9, n. 10, p. 14-pg., 2022-10-01. (15/24040-7, 09/01788-5)

DA SILVA, WAGNER RAFAEL; SILVEIRA, LANDULFO, JR.; FERNANDES, ADRIANA BARRINHA; COSTAFELIX, R; MACHADO, JC; ALVARENGA, AV. Diagnosing Iron Deficiency Anemia by Raman Spectroscopy Analysis. XXVI BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2018, VOL. 2, v. 70, n. 2, p. 5-pg., 2019-01-01. (09/01788-5)