Arthropod-borne agents in wild Orinoco geese (Neochen jubata) in Brazil

Autor(es)

Werther, Karin ^[1] ; Luzzi, Mayara de Cassia ^[1] ; Goncalves, Luiz Ricardo ^[1] ; de Oliveira, Juliana Paula ^[1] ; Ferreira Alves Junior, Jose Roberto ^[2] ; Machado, Rosangela Zacarias ^[1] ; Andre, Marcos Rogerio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Patol Vet, FCAV, Jaboticabal, SP - Brazil

^[2] IF Goiano, Urutai, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 55, p. 30-41, DEC 2017.
• Citações Web of Science: 5

Resumo

Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Gois State, Brazil. Six geese (10%) were seropositive for Anaplasma phagocytophilum, showing titers ranging from 40 and 80. Twenty out of 62 blood samples (32.25%) were positive in nested PCR for hemosporidia (cytochrome b gene). Fifteen and five sequences shared identity with Haemoproteus and Plasmodium, respectively. Six out of 62 blood samples (9.68%) were positive in nested PCR for Babesia spp. (18S rRNA gene); one sequence showed to be closely related to Babesia vogeli. Thirty (48.38%) out of 62 Orinoco geese blood samples were positive in nested cPCR assays for Anaplasmataceae agents (16S rRNA gene): three for Anaplasma spp. and 27 for Ehrlichia. Six geese were simultaneously positive to Haemoproteus and Ehrlichia; three animals were co-positive to different Ehrlichia species/genotypes; and one goose sample was positive for both Anaplasma and Ehrlichia. The present work showed the occurrence of Ehrlichia, Anaplasma, Babesia, Plasmodium, and Haemoproteus species in free-living N. jubata in Brazil. The threat of these arthropod-borne pathogens in Orinoco goose's fitness, especially during the breading season, should be assessed in the future. (AU)

• Processo FAPESP: 15/14896-1 - Detecção e caracterização molecular de agentes das famílias Anaplasmataceae e Bartonellaceae em animais selvagens e ectoparasitas no Brasil
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/11230-1 - Estudo do perfil hematológico, bioquímico sérico e de hemoparasitos em gansos do Orinoco (Neochen jubata) de vida livre
• Beneficiário: Karin Werther
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/15688-8 - Detecção molecular de hemosporídeos, piroplasmas e agentes anaplasmataceae em gansos do Orinoco (Neochen jubata) de vida livre
• Beneficiário: Mayara de Cássia Luzzi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica