Addition of Antioxidants Myoinositol, Ferulic Acid, and Melatonin and Their Effects on Sperm Motility, Membrane Integrity, and Reactive Oxygen Species Production in Cooled Equine Semen

Autor(es)

Affonso, Fernanda J. ^[1] ; Carvalho, Henrique F. ^[1] ; Lanconi, Renata ^[1] ; Lemes, Kleber M. ^[1] ; Leite, Ticiano G. ^[1] ; Oliveira, Leticia Z. ^[1] ; Celeghini, Eneiva C. C. ^[2] ; de Arruda, Rubens P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Lab Semen Biotechnol & Androl, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Lab Teaching & Res Pathol Reprod, Ctr Biotechnol Anim Reprod, Dept Anim Reprod, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Equine Veterinary Science; v. 59, p. 57-63, DEC 2017.
• Citações Web of Science: 3

Resumo

The present study aimed to evaluate the influence of myoinositol (MYO), ferulic acid (FA), and melatonin (MEL) in equine cooled semen. Ejaculates were collected and distributed into the following four treatments: MYO, FA, MEL, and control. A skim milk-based extender was used. Samples were cooled at 5 degrees C and evaluated at 0, 4, and 8 hours after storage for motility, plasma and acrosomal membranes integrity, mitochondrial potential, and production of reactive oxygen species (ROS). Motility characteristics were not affected by treatment, except for the amplitude of lateral head displacement, which was higher in MYO (8.3 +/- 0.2) compared with the control group (7.8 +/- 0.2). No difference was observed among treatments for intact plasma membrane (%). However, the percentage of cells with intact plasma and acrosomal membranes and high mitochondrial potential was greater in the MEL (78.1 +/- 2.0) and FA groups (78.8 +/- 1.7) compared with the control group (73.8 +/- 2.0). The high mitochondrial potential (%) was also greater in groups treated with MEL (80.1 +/- 1.9) and FA (81.0 +/- 1.5) compared with the control group (76.6 +/- 2.0). In addition, percentage of cells with intact acrosome membrane was greater in MEL group (99.7 +/- 0.1) compared with all other treatments. ROS production was not affected by treatments. In conclusion, FA and MEL provided the best protection to mitochondria, acrosome, and plasma membranes, suggesting that the addition of these antioxidants to equine semen extender can improve sperm quality. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/18277-6 - Efeitos de diluidores contendo lipoproteínas de baixa densidade (LDL) e colesterol, e suas interações com o tempo de equilíbrio, na criopreservação do sêmen bovino
• Beneficiário: Ticiano Guimarães Leite
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado