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Ultrastructure of spermatozoa of spider crabs, family Mithracidae (Crustacea, Decapoda, Brachyura): Integrative analyses based on morphological and molecular data

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Autor(es):
Assugeni, Camila de O. [1, 2] ; Magalhaes, Tatiana [3] ; Bolanos, Juan A. [4] ; Tudge, Christopher C. [5] ; Mantelatto, Fernando L. [3] ; Zara, Fernando J. [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, IEAMar, Prof Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Biol Aplicada, Lab Morfol Invertebrados IML, Unesp Aquaculture Ctr Caunesp, Prof Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[3] Univ Sao Paulo, Dept Biol, LBSC, FFCLRP, Ave Bandeirantes 3900, BR-14884900 Ribeirao Preto, SP - Brazil
[4] Escuela Ciencias Aplicadas Mar, Dept Acuacultura Biol Marina, Memorian Grp Invest Carcinologia GICUDONE, Dept Acuacultura & Biol Marina, Nueva Esparta, Isla De Margari - Venezuela
[5] Amer Univ, Dept Biol, 4400 Massachusetts Ave, NW, Washington, DC 20016 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Journal of Morphology; v. 278, n. 12, p. 1628-1646, DEC 2017.
Citações Web of Science: 1
Resumo

Recent studies based on morphological and molecular data provide a new perspective concerning taxonomic aspects of the brachyuran family Mithracidae. These studies proposed a series of nominal changes and indicated that the family is actually represented by a different number and representatives of genera than previously thought. Here, we provide a comparative description of the ultrastructure of spermatozoa and spermatophores of some species of Mithracidae in a phylogenetic context. The ultrastructure of the spermatozoa and spermatophore was observed by scanning and transmission electron microscopy. The most informative morphological characters analysed were thickness of the operculum, shape of the perforatorial chamber and shape and thickness of the inner acrosomal zone. As a framework, we used a topology based on a phylogenetic analysis using mitochondrial data obtained here and from previous studies. Our results indicate that closely related species share a series of morphological characteristics of the spermatozoa. A thick operculum, for example, is a feature observed in species of the genera Amphithrax, Teleophrys, and Omalacantha in contrast to the slender operculum observed in Mithraculus and Mithrax. Amphithrax and Teleophrys have a rhomboid perforatorial chamber, while Mithraculus, Mithrax, and Omalacantha show a wider, deltoid morphology. Furthermore, our results are in agreement with recently proposed taxonomic changes including the separation of the genera Mithrax (previously Damithrax), Amphithrax (previously Mithrax) and Mithraculus, and the synonymy of Mithrax caribbaeus with Mithrax hispidus. Overall, the spermiotaxonomy of these species of Mithracidae represent a novel set of data that corroborates the most recent taxonomic revision of the family and can be used in future taxonomic and phylogenetic studies within this family. (AU)

Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
Beneficiário:Fernando Luis Medina Mantelatto
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático