Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: multifaceted evaluation with periodontitis covariants

Autor(es)

Cirelli, Thamiris ^{[1, 2]} ; Finoti, Livia S. ^{[1, 2]} ; Corbi, Samia C. T. ^{[1, 2]} ; Anovazzi, Giovana ^{[1, 2]} ; Nepomuceno, Rafael ^{[1, 2]} ; Orrico, Silvana R. P. ^[2] ; Cirelli, Joni A. ^[2] ; Mayer, Marcia P. A. ^[3] ; Scarel-Caminaga, Raquel M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Morphol, Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Oral Diag & Surg, Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 75, n. 7 OCT 2017.
• Citações Web of Science: 3

Resumo

This study aimed to evaluate the association between haplotypes in the interleukin 8 (IL8) and IL4 genes previously associated to chronic periodontitis (CP) and the levels of Aggregatibacter actinomycetemcomitans (A.a.) in subgingival sites of patients with and without CP. Moreover, multifaceted evaluations were made to search associations among patients' genetic background with the A. a. levels and previous clinical/immunological/microbiological findings. Subgingival sites (n = 596) of 104 patients were divided into susceptible to CP by the IL8 haplotype ATC/TTC (IL8+); non-susceptible to CP by the IL8 AGT/TTC (IL8-); susceptible to CP by the IL4 TCI/CCI (IL4+); protection against CP by the IL4 TTD/CTI (IL4-). Subgingival biofilm samples from diseased and healthy sites of CP patients and from control sites of health patients were obtained for absolute quantification of A.a. by quantitative real-time polymerase chain reaction. For diseased sites, samples were collected before and 45 days after periodontal treatment. The IL4 but not the IL8 haplotypes were associated with levels of A.a. (in both periods). After periodontal treatment, higher levels of A.a. were found in subgingival sites of (IL4-) patients, and higher levels of IL-4 were associated with deeper probing pockets in these same patients. Significant correlations were found among genetic (patients carrying IL8 or IL4 haplotypes), microbiological and immunological data showing the interrelationship of different factors in the CP. (AU)

• Processo FAPESP: 09/11371-4 - Investigação comparativa de dados clínicos e microbiológicos entre indivíduos suscetíveis geneticamente e não susceptíveis à periodontite
• Beneficiário: Livia Sertori Finoti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/06365-0 - Investigação de Aggregatibacter actinomycetemcomitans em indivíduos suscetíveis geneticamente e não suscetíveis à periodontite crônica
• Beneficiário: Thamiris Cirelli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/11321-7 - Investigação do caráter multifatorial da doença periodontal: comparação de dados clínicos e imunológicos entre indivíduos suscetíveis geneticamente e não suscetíveis à periodontite
• Beneficiário: Giovana Anovazzi Medeiros
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/08773-3 - Análises clínica, microbiológica e imunológica em indivíduos com suscetibilidade genética à doença periodontal
• Beneficiário: Raquel Mantuaneli Scarel Caminaga
• Modalidade de apoio: Auxílio à Pesquisa - Regular