A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Autor(es)

Santos, Adriano ^[1] ; Bueno, Paulo R. ^[1] ; Davis, Jason J. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Sao Paulo State Univ, Inst Chem, Phys Chem Dept, Nanob Grp, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Oxford, Dept Chem, South Parks Rd, Oxford OX1 3QZ - England

• Tipo de documento: Artigo Científico
• Fonte: BIOSENSORS & BIOELECTRONICS; v. 100, p. 519-525, FEB 15 2018.
• Citações Web of Science: 6

Resumo

Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance). (AU)

• Processo FAPESP: 12/22820-7 - Sistemas de detecção ultrasensíveis livre de rotulação para testes clínicos por meio de imitância eletroquímica
• Beneficiário: Eduardo Maffud Cilli
• Modalidade de apoio: Auxílio à Pesquisa - Temático